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Molecules 2011, 16(9), 7480-7487; doi:10.3390/molecules16097480

Application of Preparative High-Speed Counter-Current Chromatography for the Separation of Two Alkaloids from the Roots of Tabernaemontana catharinensis (Apocynaceae)

Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602, Campos dos Goytacazes, Rio de Janeiro, Brazil
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Received: 28 June 2011 / Revised: 16 August 2011 / Accepted: 31 August 2011 / Published: 2 September 2011
(This article belongs to the Special Issue Alkaloids: Novel Therapeutic Perspectives)
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Abstract

The methanolic extract of Tabernaemontana catharinensis (Apocynaceae) roots, which contains alkaloids with several biological activities, was separated on a preparative scale using high-speed counter-current chromatography. The optimum solvent system was found to be a mixture of CHCl3-MeOH-H2O [5:10:6 (v/v/v)] and led to a successful separation of two monoterpenic indole alkaloids, voachalotine (1) and 12-methoxy-Nb-methylvoachalotine (2) in approximately 4.0 hours. The alkaloids were all isolated at purities over 95%, and their structures were established on the basis of spectroscopic methods, including 1D and 2D NMR and EI/MS.
Keywords: counter-current chromatography; Tabernaemontana catharinensis; alkaloids counter-current chromatography; Tabernaemontana catharinensis; alkaloids
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MDPI and ACS Style

Gonçalves, M.S.; Vieira, I.J.C.; Oliveira, R.R.; Braz-Filho, R. Application of Preparative High-Speed Counter-Current Chromatography for the Separation of Two Alkaloids from the Roots of Tabernaemontana catharinensis (Apocynaceae). Molecules 2011, 16, 7480-7487.

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