Molecules 2011, 16(9), 7365-7376; doi:10.3390/molecules16097365
Article

An Emulsion Based Microarray Method to Detect the Toxin Genes of Toxin-Producing Organisms

1, 2, 2, 2 and 1,* email
Received: 21 July 2011; in revised form: 12 August 2011 / Accepted: 25 August 2011 / Published: 29 August 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Toxins produced by bacteria and fungi are one of the most important factors which may cause food contamination. The study of detection methods with high sensitivity and throughput is significant for the protection of food safety. In the present study, we coupled microarray with emulsion PCR and developed a high throughput detection method. Thirteen different gene sites which encode the common toxins of several bacteria and fungi were assayed in parallel in positive and maize samples. Conventional PCR assays were carried out for comparison. The results showed that the developed microarray method had high specificity and sensitivity. Two zearalenone-related genes were investigated in one of the ten maize samples obtained with this present method. The results indicated that the emulsion based microarray detection method was developed successfully and suggested its potential application in multiple gene site detection.
Keywords: emulsion PCR; microarray; toxins
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MDPI and ACS Style

Wang, Y.; Lu, J.; Yang, Q.; Bai, Y.; Ge, Q. An Emulsion Based Microarray Method to Detect the Toxin Genes of Toxin-Producing Organisms. Molecules 2011, 16, 7365-7376.

AMA Style

Wang Y, Lu J, Yang Q, Bai Y, Ge Q. An Emulsion Based Microarray Method to Detect the Toxin Genes of Toxin-Producing Organisms. Molecules. 2011; 16(9):7365-7376.

Chicago/Turabian Style

Wang, Ying; Lu, Jiafeng; Yang, Qi; Bai, Yunfei; Ge, Qinyu. 2011. "An Emulsion Based Microarray Method to Detect the Toxin Genes of Toxin-Producing Organisms." Molecules 16, no. 9: 7365-7376.

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