Next Article in Journal
An Emulsion Based Microarray Method to Detect the Toxin Genes of Toxin-Producing Organisms
Previous Article in Journal
Evaluation of the Effects of Mitragyna speciosa Alkaloid Extract on Cytochrome P450 Enzymes Using a High Throughput Assay
Article Menu

Article Versions

Export Article

Open AccessArticle
Molecules 2011, 16(9), 7357-7364; doi:10.3390/molecules16097357

Antibacterial Activity of Aristolochia brevipes against Multidrug-Resistant Mycobacterium tuberculosis

Laboratory of Microbiology, Biomedical Research Center of the South (IMSS), Argentina 1, Col. Centro, 62790 Xochitepec, Morelos, Mexico
Immunochemistry Laboratory II, Department of Immunology, National School of Sciences Bilogy (IPN), Prolongación Carpio y Plan de Ayala S/N, Col. Casco de Santo Tomás, 11340 Mexico, D.F., Mexico
Chemical Research Center, University of Morelos (UAEM), Av. Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, Mexico
Author to whom correspondence should be addressed.
Received: 20 July 2011 / Revised: 18 August 2011 / Accepted: 23 August 2011 / Published: 29 August 2011
(This article belongs to the Section Natural Products)
Download PDF [353 KB, uploaded 18 June 2014]


The increased incidence of Multidrug-Resistant Mycobacterium tuberculosis (MDR-MT) requires the search for alternative antimycobacterial drugs. The main aim of this study was to evaluate the dichloromethane extract from Aristolochia brevipes (Rhizoma) and the compounds isolated from this extract against several mycobacterial strains, sensitive, resistant (monoresistant), and clinical isolates (multidrug-resistant), using the alamarBlue™ microassay. The extract was fractionated by column chromatography, yielding the following eight major compounds: (1) 6α-7-dehydro-N-formylnornantenine; (2) E/Z-N-formylnornantenine; (3) 7,9-dimethoxytariacuripyrone; (4) 9-methoxy-tariacuripyrone; (5) aristololactam I; (6) β-sitosterol; (7) stigmasterol; and (8) 3-hydroxy-α-terpineol. The structures of these compounds were elucidated by 1H- and 13C- (1D and 2D) Nuclear Magnetic Resonance (NMR) spectroscopy. This study demonstrates that the dichloromethane extract (rhizome) of A. brevipes possesses strong in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv (Minimum Inhibitory Concentration value [MIC], 12.5 µg/mL). The most active compound against all mycobacterial strains tested was the compound aristolactam I (5), with MIC values ranging between 12.5 and 25 µg/mL. To our knowledge, this the first report of antimycobacterial activity in this plant.
Keywords: Mycobacterium tuberculosis; Multidrug-resistant (MDR); Aristolochia brevipes; alamarBlue™ microassay; medicinal plant Mycobacterium tuberculosis; Multidrug-resistant (MDR); Aristolochia brevipes; alamarBlue™ microassay; medicinal plant
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Navarro-García, V.M.; Luna-Herrera, J.; Rojas-Bribiesca, M.G.; Álvarez-Fitz, P.; Ríos, M.Y. Antibacterial Activity of Aristolochia brevipes against Multidrug-Resistant Mycobacterium tuberculosis. Molecules 2011, 16, 7357-7364.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top