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Molecules 2011, 16(9), 7357-7364; doi:10.3390/molecules16097357
Article

Antibacterial Activity of Aristolochia brevipes against Multidrug-Resistant Mycobacterium tuberculosis

1,* , 2, 1, 1 and 3
1 Laboratory of Microbiology, Biomedical Research Center of the South (IMSS), Argentina 1, Col. Centro, 62790 Xochitepec, Morelos, Mexico 2 Immunochemistry Laboratory II, Department of Immunology, National School of Sciences Bilogy (IPN), Prolongación Carpio y Plan de Ayala S/N, Col. Casco de Santo Tomás, 11340 Mexico, D.F., Mexico 3 Chemical Research Center, University of Morelos (UAEM), Av. Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, Mexico
* Author to whom correspondence should be addressed.
Received: 20 July 2011 / Revised: 18 August 2011 / Accepted: 23 August 2011 / Published: 29 August 2011
(This article belongs to the Section Metabolites)
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Abstract

The increased incidence of Multidrug-Resistant Mycobacterium tuberculosis (MDR-MT) requires the search for alternative antimycobacterial drugs. The main aim of this study was to evaluate the dichloromethane extract from Aristolochia brevipes (Rhizoma) and the compounds isolated from this extract against several mycobacterial strains, sensitive, resistant (monoresistant), and clinical isolates (multidrug-resistant), using the alamarBlue™ microassay. The extract was fractionated by column chromatography, yielding the following eight major compounds: (1) 6α-7-dehydro-N-formylnornantenine; (2) E/Z-N-formylnornantenine; (3) 7,9-dimethoxytariacuripyrone; (4) 9-methoxy-tariacuripyrone; (5) aristololactam I; (6) β-sitosterol; (7) stigmasterol; and (8) 3-hydroxy-α-terpineol. The structures of these compounds were elucidated by 1H- and 13C- (1D and 2D) Nuclear Magnetic Resonance (NMR) spectroscopy. This study demonstrates that the dichloromethane extract (rhizome) of A. brevipes possesses strong in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv (Minimum Inhibitory Concentration value [MIC], 12.5 µg/mL). The most active compound against all mycobacterial strains tested was the compound aristolactam I (5), with MIC values ranging between 12.5 and 25 µg/mL. To our knowledge, this the first report of antimycobacterial activity in this plant.
Keywords: Mycobacterium tuberculosis; Multidrug-resistant (MDR); Aristolochia brevipes; alamarBlue™ microassay; medicinal plant Mycobacterium tuberculosis; Multidrug-resistant (MDR); Aristolochia brevipes; alamarBlue™ microassay; medicinal plant
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Navarro-García, V.M.; Luna-Herrera, J.; Rojas-Bribiesca, M.G.; Álvarez-Fitz, P.; Ríos, M.Y. Antibacterial Activity of Aristolochia brevipes against Multidrug-Resistant Mycobacterium tuberculosis. Molecules 2011, 16, 7357-7364.

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