Molecules 2011, 16(9), 7357-7364; doi:10.3390/molecules16097357
Article

Antibacterial Activity of Aristolochia brevipes against Multidrug-Resistant Mycobacterium tuberculosis

1,* email, 2, 1, 1 and 3
Received: 20 July 2011; in revised form: 18 August 2011 / Accepted: 23 August 2011 / Published: 29 August 2011
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The increased incidence of Multidrug-Resistant Mycobacterium tuberculosis (MDR-MT) requires the search for alternative antimycobacterial drugs. The main aim of this study was to evaluate the dichloromethane extract from Aristolochia brevipes (Rhizoma) and the compounds isolated from this extract against several mycobacterial strains, sensitive, resistant (monoresistant), and clinical isolates (multidrug-resistant), using the alamarBlue™ microassay. The extract was fractionated by column chromatography, yielding the following eight major compounds: (1) 6α-7-dehydro-N-formylnornantenine; (2) E/Z-N-formylnornantenine; (3) 7,9-dimethoxytariacuripyrone; (4) 9-methoxy-tariacuripyrone; (5) aristololactam I; (6) β-sitosterol; (7) stigmasterol; and (8) 3-hydroxy-α-terpineol. The structures of these compounds were elucidated by 1H- and 13C- (1D and 2D) Nuclear Magnetic Resonance (NMR) spectroscopy. This study demonstrates that the dichloromethane extract (rhizome) of A. brevipes possesses strong in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv (Minimum Inhibitory Concentration value [MIC], 12.5 µg/mL). The most active compound against all mycobacterial strains tested was the compound aristolactam I (5), with MIC values ranging between 12.5 and 25 µg/mL. To our knowledge, this the first report of antimycobacterial activity in this plant.
Keywords: Mycobacterium tuberculosis; Multidrug-resistant (MDR); Aristolochia brevipes; alamarBlue™ microassay; medicinal plant
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MDPI and ACS Style

Navarro-García, V.M.; Luna-Herrera, J.; Rojas-Bribiesca, M.G.; Álvarez-Fitz, P.; Ríos, M.Y. Antibacterial Activity of Aristolochia brevipes against Multidrug-Resistant Mycobacterium tuberculosis. Molecules 2011, 16, 7357-7364.

AMA Style

Navarro-García VM, Luna-Herrera J, Rojas-Bribiesca MG, Álvarez-Fitz P, Ríos MY. Antibacterial Activity of Aristolochia brevipes against Multidrug-Resistant Mycobacterium tuberculosis. Molecules. 2011; 16(9):7357-7364.

Chicago/Turabian Style

Navarro-García, Víctor Manuel; Luna-Herrera, Julieta; Rojas-Bribiesca, Ma. Gabriela; Álvarez-Fitz, Patricia; Ríos, María Yolanda. 2011. "Antibacterial Activity of Aristolochia brevipes against Multidrug-Resistant Mycobacterium tuberculosis." Molecules 16, no. 9: 7357-7364.

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