Molecules 2011, 16(8), 6378-6395; doi:10.3390/molecules16086378
Article

Antioxidant Capacities and Total Phenolic Contents Increase with Gamma Irradiation in Two Types of Malaysian Honey

Saba Zuhair Hussein 1 email, Kamaruddin Mohd Yusoff 2 email, Suzana Makpol 1 email and Yasmin Anum Mohd Yusof 1,* email
1 Department of Biochemistry, Faculty of Medicine, University Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia 2 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
* Author to whom correspondence should be addressed.
Received: 25 May 2011; in revised form: 21 July 2011 / Accepted: 25 July 2011 / Published: 27 July 2011
(This article belongs to the Section Natural Products)
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Abstract: Two types of monofloral Malaysian honey (Gelam and Nenas) were analyzed to determine their antioxidant activities and total phenolic and flavonoid contents, with and without gamma irradiation. Our results showed that both types of honey can scavenge free radicals and exhibit high antioxidant-reducing power; however, Gelam honey exhibited higher antioxidant activity (p < 0.05) than Nenas honey, which is in good correlation (r = 0.9899) with its phenolic contents. Interestingly, we also noted that both irradiated honeys have higher antioxidant activities and total phenolic and flavonoid contents compared to nonirradiated honeys by Folin-Ciocalteu and UV-spectrophotometry methods, respectively. However, HPLC analysis for phenolic compounds showed insignificant increase between irradiated and nonirradiated honeys. The phenolic compounds such as: caffeic acid, chlorogenic acid, ellagic acid, p- coumaric acid, quercetin and hesperetin as indicated by HPLC method were found to be higher in Gelam honey versus Nenas honey. In conclusion, irradiation of honey causes enhanced antioxidant activities and flavonoid compounds.
Keywords: Gelam honey; Nenas honey; antioxidant activity; phenolics; gamma irradiation

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MDPI and ACS Style

Hussein, S.Z.; Yusoff, K.M.; Makpol, S.; Yusof, Y.A.M. Antioxidant Capacities and Total Phenolic Contents Increase with Gamma Irradiation in Two Types of Malaysian Honey. Molecules 2011, 16, 6378-6395.

AMA Style

Hussein SZ, Yusoff KM, Makpol S, Yusof YAM. Antioxidant Capacities and Total Phenolic Contents Increase with Gamma Irradiation in Two Types of Malaysian Honey. Molecules. 2011; 16(8):6378-6395.

Chicago/Turabian Style

Hussein, Saba Zuhair; Yusoff, Kamaruddin Mohd; Makpol, Suzana; Yusof, Yasmin Anum Mohd. 2011. "Antioxidant Capacities and Total Phenolic Contents Increase with Gamma Irradiation in Two Types of Malaysian Honey." Molecules 16, no. 8: 6378-6395.

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