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Molecules 2011, 16(7), 5905-5915; doi:10.3390/molecules16075905
Article

HPLC Separation of All Aldopentoses and Aldohexoses on an Anion-Exchange Stationary Phase Prepared from Polystyrene-Based Copolymer and Diamine: The Effect of NaOH Eluent Concentration

1, 2, 1, 2 and 2,*
Received: 13 June 2011; in revised form: 4 July 2011 / Accepted: 7 July 2011 / Published: 14 July 2011
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Abstract: To investigate the separations of all aldopentoses (ribose, arabinose, xylose and lyxose) and aldohexoses (glucose, galactose, allose, altrose, mannose, gulose, idose and talose) on the D6 stationary phase prepared by the reaction of chloromethylated styrene-divinylbenzene copolymer and N,N,N’,N’-tetramethyl-1,6-diaminohexane, we examined the effect of varying the concentration of the NaOH eluent on the elution orders. Separations of these aldoses were achieved using a 20 mM NaOH eluent. The elution behaviors of the aldoses were probably due to not only the individual pKa values, but also the chemical structures of the cyclic aldoses.
Keywords: carbohydrate; aldose; aldopentose; aldohexose; high-performance anion-exchange chromatography carbohydrate; aldose; aldopentose; aldohexose; high-performance anion-exchange chromatography
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Inoue, K.; Kitahara, K.-I.; Aikawa, Y.; Arai, S.; Masuda-Hanada, T. HPLC Separation of All Aldopentoses and Aldohexoses on an Anion-Exchange Stationary Phase Prepared from Polystyrene-Based Copolymer and Diamine: The Effect of NaOH Eluent Concentration. Molecules 2011, 16, 5905-5915.

AMA Style

Inoue K, Kitahara K-I, Aikawa Y, Arai S, Masuda-Hanada T. HPLC Separation of All Aldopentoses and Aldohexoses on an Anion-Exchange Stationary Phase Prepared from Polystyrene-Based Copolymer and Diamine: The Effect of NaOH Eluent Concentration. Molecules. 2011; 16(7):5905-5915.

Chicago/Turabian Style

Inoue, Kadumi; Kitahara, Kei-ichi; Aikawa, Yoshihiro; Arai, Sadao; Masuda-Hanada, Takako. 2011. "HPLC Separation of All Aldopentoses and Aldohexoses on an Anion-Exchange Stationary Phase Prepared from Polystyrene-Based Copolymer and Diamine: The Effect of NaOH Eluent Concentration." Molecules 16, no. 7: 5905-5915.


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