Next Article in Journal
Industrial Aplication of Catalytic Systems for n-Heptane Isomerization
Previous Article in Journal
Biocatalytic Resolution of Enantiomeric Mixtures of 1-Aminoethanephosphonic Acid
Molecules 2011, 16(7), 5905-5915; doi:10.3390/molecules16075905
Article

HPLC Separation of All Aldopentoses and Aldohexoses on an Anion-Exchange Stationary Phase Prepared from Polystyrene-Based Copolymer and Diamine: The Effect of NaOH Eluent Concentration

1, 2, 1, 2 and 2,*
1 Department of Human Environmental Sciences, Ochanomizu University, 2-1-1 Otuka, Bunkyo-ku, Tokyo 112-8610, Japan 2 Department of Chemistry, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan
* Author to whom correspondence should be addressed.
Received: 13 June 2011 / Revised: 4 July 2011 / Accepted: 7 July 2011 / Published: 14 July 2011
Download PDF [583 KB, 18 June 2014; original version 18 June 2014]

Abstract

To investigate the separations of all aldopentoses (ribose, arabinose, xylose and lyxose) and aldohexoses (glucose, galactose, allose, altrose, mannose, gulose, idose and talose) on the D6 stationary phase prepared by the reaction of chloromethylated styrene-divinylbenzene copolymer and N,N,N’,N’-tetramethyl-1,6-diaminohexane, we examined the effect of varying the concentration of the NaOH eluent on the elution orders. Separations of these aldoses were achieved using a 20 mM NaOH eluent. The elution behaviors of the aldoses were probably due to not only the individual pKa values, but also the chemical structures of the cyclic aldoses.
Keywords: carbohydrate; aldose; aldopentose; aldohexose; high-performance anion-exchange chromatography carbohydrate; aldose; aldopentose; aldohexose; high-performance anion-exchange chromatography
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Inoue, K.; Kitahara, K.-I.; Aikawa, Y.; Arai, S.; Masuda-Hanada, T. HPLC Separation of All Aldopentoses and Aldohexoses on an Anion-Exchange Stationary Phase Prepared from Polystyrene-Based Copolymer and Diamine: The Effect of NaOH Eluent Concentration. Molecules 2011, 16, 5905-5915.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert