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Identification of N6,N6-Dimethyladenosine in Transfer RNA from Mycobacterium bovis Bacille Calmette-Guérin
Department of Chemistry, 56-787, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Department of Biological Engineering, 56-787, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Singapore-MIT Alliance for Research and Technology, Center for Life Sciences #05-06, 28 Medical Drive, Singapore 117456, Singapore
Department of Microbiology, National University of Singapore, #03-05 28 Medical Drive, Singapore 117456, Singapore
* Author to whom correspondence should be addressed.
Received: 4 May 2011; in revised form: 3 June 2011 / Accepted: 10 June 2011 / Published: 21 June 2011
Abstract: There are more than 100 different ribonucleoside structures incorporated as post-transcriptional modifications, mainly in tRNA and rRNA of both prokaryotes and eukaryotes, and emerging evidence suggests that these modifications function as a system in the translational control of cellular responses. However, our understanding of this system is hampered by the paucity of information about the complete set of RNA modifications present in individual organisms. To this end, we have employed a chromatography-coupled mass spectrometric approach to define the spectrum of modified ribonucleosides in microbial species, starting with Mycobacterium bovis BCG. This approach revealed a variety of ribonucleoside candidates in tRNA from BCG, of which 12 were definitively identified based on comparisons to synthetic standards and 5 were tentatively identified by exact mass comparisons to RNA modification databases. Among the ribonucleosides observed in BCG tRNA was one not previously described in tRNA, which we have now characterized as N6,N6-dimethyladenosine.
Keywords: tRNA; ribonucleoside modification; Mycobacteriaum bovis; BCG
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Chan, C.T.; Chionh, Y.H.; Ho, C.-H.; Lim, K.S.; Babu, I.R.; Ang, E.; Wenwei, L.; Alonso, S.; Dedon, P.C. Identification of N6,N6-Dimethyladenosine in Transfer RNA from Mycobacterium bovis Bacille Calmette-Guérin. Molecules 2011, 16, 5168-5181.
Chan CT, Chionh YH, Ho C-H, Lim KS, Babu IR, Ang E, Wenwei L, Alonso S, Dedon PC. Identification of N6,N6-Dimethyladenosine in Transfer RNA from Mycobacterium bovis Bacille Calmette-Guérin. Molecules. 2011; 16(6):5168-5181.
Chan, Clement T.; Chionh, Yok Hian; Ho, Chia-Hua; Lim, Kok Seong; Babu, I. Ramesh; Ang, Emily; Wenwei, Lin; Alonso, Sylvie; Dedon, Peter C. 2011. "Identification of N6,N6-Dimethyladenosine in Transfer RNA from Mycobacterium bovis Bacille Calmette-Guérin." Molecules 16, no. 6: 5168-5181.