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Anti-Migration Effects of Gekko Sulfated Glycopeptide on Human Hepatoma SMMC-7721 Cells
Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital, Huan-Hu-Xi-Road, He-Xi-District, Tianjin 300060, China
Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China
State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK), Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong SAR, China
* Author to whom correspondence should be addressed.
Received: 30 May 2011; in revised form: 11 June 2011 / Accepted: 14 June 2011 / Published: 16 June 2011
Abstract: Gekko swinhonis Guenther has been used as an anti-cancer drug in traditional Chinese medicine for hundreds of years. Previous studies showed that the Gekko sulfated polysaccharide-protein complex suppressed the proliferation and migration of hepatoma cells. Gekko sulfated glycopeptide α was obtained from Gekko sulfated polysaccharide-protein complex using papain hydrolysis. Gekko sulfated glycopeptide α inhibited the proliferation and migration of SMMC-7721 cells. The secretion of IL-8 and the concentration of intracellular calcium were decreased after Gekko sulfated glycopeptide α exposure. SMMC-7721 cells in the control group showed abnormal features, with a polygonal shape, whereas this changed to a spindle shape after the treatment with Gekko sulfated glycopeptide α. Actin ﬁlaments were distributed diffusely along the cell membrane in control cells, whereas those were polymerized and preferentially accumulated in the cytoplasm of treated cells. Microtubules distributed in the cytoplasm of untreated cells were located diffusely whereas those in treated cells were polymerized. Therefore, Gekko sulfated glycopeptide α inhibit the migration of hepatoma cells via reducing the secretion of IL-8 and the concentration of intracellular calcium, as well as regulating the reorganization of cytoskeleton.
Keywords: Gekko swinhonis Guenther; sulfated glycopeptide; hepatocellular carcinoma; migration
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MDPI and ACS Style
Wu, X.-Z.; Chen, D.; Han, X.-Q. Anti-Migration Effects of Gekko Sulfated Glycopeptide on Human Hepatoma SMMC-7721 Cells. Molecules 2011, 16, 4958-4970.
Wu X-Z, Chen D, Han X-Q. Anti-Migration Effects of Gekko Sulfated Glycopeptide on Human Hepatoma SMMC-7721 Cells. Molecules. 2011; 16(6):4958-4970.
Wu, Xiong-Zhi; Chen, Dan; Han, Xiao-Qiang. 2011. "Anti-Migration Effects of Gekko Sulfated Glycopeptide on Human Hepatoma SMMC-7721 Cells." Molecules 16, no. 6: 4958-4970.