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Molecules 2011, 16(6), 4836-4849; doi:10.3390/molecules16064836

The Immuno-Regulatory Effects of Schisandra chinensis and Its Constituents on Human Monocytic Leukemia Cells

1
Department of Internal Medicine, Ho-Ping Branch, Taipei City Hospital, Taipei 100, Taiwan
2
Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan
3
School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan
4
Department of Microbiology and Immunology, Taipei Medical University, Taipei 110, Taiwan
5
Center for Reproductive Medicine & Sciences, Taipei Medical University Hospital, Taipei 110, Taiwan
*
Author to whom correspondence should be addressed.
Received: 19 April 2011 / Revised: 7 June 2011 / Accepted: 9 June 2011 / Published: 10 June 2011
(This article belongs to the Section Natural Products)
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Abstract

Many diseases occur when the immune system is weakened. Intracellular signals activate immuno-responsive cells to produce cytokines that modulate the immune response. Schisandra chinensis has been used traditionally to treat general fatigue, neurasthenia, and spontaneous sweating. In the present study, the effect of constituents of S. chinensis on cytokine release by human monocytic leukemia cells (THP-1) was tested using microparticle-based flow cytometric analysis. Two major lignans, schizandrin (Sch) and gomisin A (Gom A), were identified and shown to induce interleukin (IL)-8, macrophage inflammatory protein-1β (MIP-1β), and granulocyte-macrophage-colony stimulating factor (GM-CSF) release by THP-1 cells. By reverse transcription polymerase chain reaction (RT-PCR) or quantitative real-time PCR, there was a dose-dependent increase of IL-8, MIP-1β and GM-CSF mRNA levels. Thus, Sch and Gom A from S. chinensis enhance cytokine release by THP-1 cells and this effect occurs through mRNA upregulation. Upregulation of MIP-1β and GM-CSF in particular may have clinical applications. Therefore, S. chinensis may be therapeutically beneficial by promoting humoral and cell-mediated immune responses.
Keywords: schizandrin; gomisin A; macrophage inflammatory protein-1b; granulocyte-macrophage-colony stimulating factor; polymerase chain reaction schizandrin; gomisin A; macrophage inflammatory protein-1b; granulocyte-macrophage-colony stimulating factor; polymerase chain reaction
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Lin, R.-D.; Mao, Y.-W.; Leu, S.-J.; Huang, C.-Y.; Lee, M.-H. The Immuno-Regulatory Effects of Schisandra chinensis and Its Constituents on Human Monocytic Leukemia Cells. Molecules 2011, 16, 4836-4849.

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