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The Immuno-Regulatory Effects of Schisandra chinensis and Its Constituents on Human Monocytic Leukemia Cells
Department of Internal Medicine, Ho-Ping Branch, Taipei City Hospital, Taipei 100, Taiwan
Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan
School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan
Department of Microbiology and Immunology, Taipei Medical University, Taipei 110, Taiwan
Center for Reproductive Medicine & Sciences, Taipei Medical University Hospital, Taipei 110, Taiwan
* Author to whom correspondence should be addressed.
Received: 19 April 2011; in revised form: 7 June 2011 / Accepted: 9 June 2011 / Published: 10 June 2011
Abstract: Many diseases occur when the immune system is weakened. Intracellular signals activate immuno-responsive cells to produce cytokines that modulate the immune response. Schisandra chinensis has been used traditionally to treat general fatigue, neurasthenia, and spontaneous sweating. In the present study, the effect of constituents of S. chinensis on cytokine release by human monocytic leukemia cells (THP-1) was tested using microparticle-based flow cytometric analysis. Two major lignans, schizandrin (Sch) and gomisin A (Gom A), were identified and shown to induce interleukin (IL)-8, macrophage inflammatory protein-1β (MIP-1β), and granulocyte-macrophage-colony stimulating factor (GM-CSF) release by THP-1 cells. By reverse transcription polymerase chain reaction (RT-PCR) or quantitative real-time PCR, there was a dose-dependent increase of IL-8, MIP-1β and GM-CSF mRNA levels. Thus, Sch and Gom A from S. chinensis enhance cytokine release by THP-1 cells and this effect occurs through mRNA upregulation. Upregulation of MIP-1β and GM-CSF in particular may have clinical applications. Therefore, S. chinensis may be therapeutically beneficial by promoting humoral and cell-mediated immune responses.
Keywords: schizandrin; gomisin A; macrophage inflammatory protein-1b; granulocyte-macrophage-colony stimulating factor; polymerase chain reaction
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Lin, R.-D.; Mao, Y.-W.; Leu, S.-J.; Huang, C.-Y.; Lee, M.-H. The Immuno-Regulatory Effects of Schisandra chinensis and Its Constituents on Human Monocytic Leukemia Cells. Molecules 2011, 16, 4836-4849.
Lin R-D, Mao Y-W, Leu S-J, Huang C-Y, Lee M-H. The Immuno-Regulatory Effects of Schisandra chinensis and Its Constituents on Human Monocytic Leukemia Cells. Molecules. 2011; 16(6):4836-4849.
Lin, Rong-Dih; Mao, Yi-Wen; Leu, Sy-Jye; Huang, Ching-Yi; Lee, Mei-Hsien. 2011. "The Immuno-Regulatory Effects of Schisandra chinensis and Its Constituents on Human Monocytic Leukemia Cells." Molecules 16, no. 6: 4836-4849.