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Molecules 2011, 16(3), 2507-2518; doi:10.3390/molecules16032507

Screening of Natural Organic Volatiles from Prunus mahaleb L. Honey: Coumarin and Vomifoliol as Nonspecific Biomarkers

1
Faculty of Chemistry and Technology, University of Split, N. Tesle 10/V, 21000 Split, Croatia
2
Marko Marulić Polytechnic in Knin, P. Krešimira IV 30, 22300 Knin, Croatia
3
Department of Biotechnology, University of Rijeka, S. Krautzeka bb, 51000 Rijeka, Croatia
*
Author to whom correspondence should be addressed.
Received: 18 February 2011 / Revised: 15 March 2011 / Accepted: 15 March 2011 / Published: 16 March 2011
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Abstract

Headspace solid-phase microextraction (HS-SPME; PDMS/DVB fibre) and ultrasonic solvent extraction (USE; solvent A: pentane and diethyl ether (1:2 v/v), solvent B: dichloromethane) followed by gas chromatography and mass spectrometry (GC, GC-MS) were used for the analysis of Prunus mahaleb L. honey samples. Screening was focused toward chemical composition of natural organic volatiles to determine if it is useful as a method of determining honey-sourcing. A total of 34 compounds were identified in the headspace and 49 in the extracts that included terpenes, norisoprenoids and benzene derivatives, followed by minor percentages of aliphatic compounds and furan derivatives. High vomifoliol percentages (10.7%–24.2%) in both extracts (dominant in solvent B) and coumarin (0.3%–2.4%) from the extracts (more abundant in solvent A) and headspace (0.9%–1.8%) were considered characteristic for P. mahaleb honey and highlighted as potential nonspecific biomarkers of the honey’s botanical origin. In addition, comparison with P. mahaleb flowers, leaves, bark and wood volatiles from our previous research revealed common compounds among norisoprenoids and benzene derivatives.
Keywords: Prunus mahaleb L. honey; headspace-solid-phase microextraction (HS-SPME); ultrasonic solvent extraction (USE); gas chromatography and mass spectrometry (GC, GC-MS); coumarin; vomifoliol Prunus mahaleb L. honey; headspace-solid-phase microextraction (HS-SPME); ultrasonic solvent extraction (USE); gas chromatography and mass spectrometry (GC, GC-MS); coumarin; vomifoliol
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Jerković, I.; Marijanović, Z.; Staver, M.M. Screening of Natural Organic Volatiles from Prunus mahaleb L. Honey: Coumarin and Vomifoliol as Nonspecific Biomarkers. Molecules 2011, 16, 2507-2518.

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