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Molecules 2011, 16(2), 1201-1210; doi:10.3390/molecules16021201
Article

Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography

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Received: 2 December 2010; in revised form: 23 January 2011 / Accepted: 25 January 2011 / Published: 27 January 2011
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Abstract: Three anthraquinones—emodin, chrysophanol, and physcion—were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n-hexane–ethanol–water (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by 1H-NMR and 13C-NMR analysis. This is the first time these anthraquinones have been obtained from R. japonicus by HSCCC.
Keywords: Rumex japonicus Houtt.; anthraquinones; high-speed counter-current chromatography (HSCCC); emodin; chrysophanol; physcion Rumex japonicus Houtt.; anthraquinones; high-speed counter-current chromatography (HSCCC); emodin; chrysophanol; physcion
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Guo, S.; Feng, B.; Zhu, R.; Ma, J.; Wang, W. Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography. Molecules 2011, 16, 1201-1210.

AMA Style

Guo S, Feng B, Zhu R, Ma J, Wang W. Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography. Molecules. 2011; 16(2):1201-1210.

Chicago/Turabian Style

Guo, Shuying; Feng, Bo; Zhu, Ruonan; Ma, Jiankang; Wang, Wei. 2011. "Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography." Molecules 16, no. 2: 1201-1210.


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