Abstract: Three anthraquinones—emodin, chrysophanol, and physcion—were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n-hexane–ethanol–water (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by 1H-NMR and 13C-NMR analysis. This is the first time these anthraquinones have been obtained from R. japonicus by HSCCC.
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Guo, S.; Feng, B.; Zhu, R.; Ma, J.; Wang, W. Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography. Molecules 2011, 16, 1201-1210.
Guo S, Feng B, Zhu R, Ma J, Wang W. Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography. Molecules. 2011; 16(2):1201-1210.
Guo, Shuying; Feng, Bo; Zhu, Ruonan; Ma, Jiankang; Wang, Wei. 2011. "Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography." Molecules 16, no. 2: 1201-1210.