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Molecules 2011, 16(2), 1201-1210; doi:10.3390/molecules16021201
Article

Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography

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1,*  and 2,*
1 College of Pharmacy, Jilin Medical College, Jilin 132013, China 2 Institute of Phytochemistry, Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China
* Authors to whom correspondence should be addressed.
Received: 2 December 2010 / Revised: 23 January 2011 / Accepted: 25 January 2011 / Published: 27 January 2011
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Abstract

Three anthraquinones—emodin, chrysophanol, and physcion—were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n-hexane–ethanol–water (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by 1H-NMR and 13C-NMR analysis. This is the first time these anthraquinones have been obtained from R. japonicus by HSCCC.
Keywords: Rumex japonicus Houtt.; anthraquinones; high-speed counter-current chromatography (HSCCC); emodin; chrysophanol; physcion Rumex japonicus Houtt.; anthraquinones; high-speed counter-current chromatography (HSCCC); emodin; chrysophanol; physcion
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Guo, S.; Feng, B.; Zhu, R.; Ma, J.; Wang, W. Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography. Molecules 2011, 16, 1201-1210.

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