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Molecules 2011, 16(11), 9600-9610; doi:10.3390/molecules16119600
Article

Interaction of the Main Components from the Traditional Chinese Drug Pair Chaihu-Shaoyao Based on Rat Intestinal Absorption

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Received: 18 October 2011; in revised form: 8 November 2011 / Accepted: 10 November 2011 / Published: 17 November 2011
(This article belongs to the Section Organic Synthesis)
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Abstract: The Chaihu-Shaoyao drug pair (Bupleuri Radix and Paeoniae Radix Alba) which is a traditional Chinese drug pair, has been widely used for anti-inflammatory purposes. Saikosaponin a (SSA), saikosaponin d (SSD) and paeoniflorin are identified as the main components in the pair. The present study focused on the interaction of the main components based on investigating their intestinal absorption using a four-site perfused rat intestinal model in order to clarify the mechanism of the compatibility of Chaihu-Shaoyao. The concentrations of SSA, SSD and paeoniflorin in the intestinal perfusate were determined by LC/MS or UPLC (Ultra Performance Liquid Chromatography) methods, followed by P*eff (effective permeability) and 10% ABS (the percent absorption of 10 cm of intestine) calculations. The results showed that all of the three main components displayed very low permeabilities(P*eff < 0.4), which implied their poor absorption in the rat intestine. The absorption levels of SSA and SSD were similar in intestine and higher in ileum than those in other intestinal regions in the decreasing order: colon, jejunum and duodenum. However, there is no significant difference in the absorption of paeoniflorin in the four segments (P < 0.05). The P*eff values of paeoniflorin exhibited an almost 2.11-fold or 1.90-fold increase in ileum when it was co-administrated with SSA and SSD, as well as 2.42-, 2.18-fold increase in colon, respectively, whereas the absorptions of SSA and SSD were not influenced by paeoniflorin. In conclusion, SSA and SSD could promote the absorption of paeoniflorin. To some extent this might explain the nature of the compatibility mechanisms of composite formulae in TCMs.
Keywords: saikosaponin a; saikosaponin d; paeoniflorin; rat intestinal perfusion model; interaction saikosaponin a; saikosaponin d; paeoniflorin; rat intestinal perfusion model; interaction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chen, Y.; Wang, J.; Yuan, L.; Zhou, L.; Jia, X.; Tan, X. Interaction of the Main Components from the Traditional Chinese Drug Pair Chaihu-Shaoyao Based on Rat Intestinal Absorption. Molecules 2011, 16, 9600-9610.

AMA Style

Chen Y, Wang J, Yuan L, Zhou L, Jia X, Tan X. Interaction of the Main Components from the Traditional Chinese Drug Pair Chaihu-Shaoyao Based on Rat Intestinal Absorption. Molecules. 2011; 16(11):9600-9610.

Chicago/Turabian Style

Chen, Yan; Wang, Jinyan; Yuan, Ling; Zhou, Lei; Jia, Xiaobin; Tan, Xiaobin. 2011. "Interaction of the Main Components from the Traditional Chinese Drug Pair Chaihu-Shaoyao Based on Rat Intestinal Absorption." Molecules 16, no. 11: 9600-9610.



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