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Effect of Ginkgo Biloba Extract 50 on Immunity and Antioxidant Enzyme Activities in Ischemia Reperfusion Rats
Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China
Department of Cardiology, ChenCang Hospital, BaoJi 721300, China
Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China
* Author to whom correspondence should be addressed.
Received: 8 September 2011; in revised form: 18 October 2011 / Accepted: 19 October 2011 / Published: 2 November 2011
Abstract: The aim of the study was to investigate the effect of Ginkgo biloba extract 50 (GBE50), a well-known natural antioxidant, against immunity and antioxidant enzyme activities in ischemia reperfusion (IR) rats. Rats were then divided into six groups fed for 15 days with the same diet: three groups (IV, V, VI) were treated by different doses of GBE50 suspension [20, 40, or 60 mg/kg body weight by oral gavage every day at a fixed time (10.00 a.m.)] (equal to 5, 10 and 20 times, respectively, the maximum recommended human dose), and three groups (I, II, III) were untreated. At the end of the experiment, rats’ hearts were subjected to 30 min of ischemia followed by 90 min of reperfusion. Results showed that IR significantly enhanced heart rate, S-T height, myocardium (myeloperoxidase) MPO activity and blood interleukin-8 (IL-8), tumor necrosis factor Alpha (TNF-a), interleukin-1β (IL-1β) levels, blood aspartate transaminase (AST), lactate dehydrogenase (LDH), and creatinine kinase (CK) activities, reduced myocardium sodium-potassium adenosine triphosphatase (Na+-K+-ATPase), calcium-magnesium adenosine triphosphatase (Ca2+-Mg2+-ATPase) activities and antioxidant enzyme activities in IR group (III) compared to sham control group (II). Pretreatment of GBE50 markedly significantly reduced heart rate, S-T height, myocardium MPO activity and blood IL-8, TNF-a, IL-1β levels, blood AST, LDH, and CK activities, enhanced myocardium Na+-K+-ATPase, Ca2+-Mg2+-ATPase activities and antioxidant enzyme activities in IR group (II) compared to IR group (III). The results suggested that the GBE50 may reduce the oxidative stress in the reperfused myocardium, and increased immunity and antioxidant activities in IR rats.
Keywords: myocardial ischemia-reperfusion; antioxidant; immunity; rat; GBE50
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MDPI and ACS Style
Lu, S.; Guo, X.; Zhao, P. Effect of Ginkgo Biloba Extract 50 on Immunity and Antioxidant Enzyme Activities in Ischemia Reperfusion Rats. Molecules 2011, 16, 9194-9206.
Lu S, Guo X, Zhao P. Effect of Ginkgo Biloba Extract 50 on Immunity and Antioxidant Enzyme Activities in Ischemia Reperfusion Rats. Molecules. 2011; 16(11):9194-9206.
Lu, Shaoping; Guo, Xia; Zhao, Pinting. 2011. "Effect of Ginkgo Biloba Extract 50 on Immunity and Antioxidant Enzyme Activities in Ischemia Reperfusion Rats." Molecules 16, no. 11: 9194-9206.