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• Bautista, M
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Molecules 2011, 16(10), 8319-8331; doi:10.3390/molecules16108319

Article

Role of Kupffer Cells in Thioacetamide-Induced Cell Cycle Dysfunction

Mirandeli Bautista ^1,* , David Andres ² , María Cascales ² , José A. Morales-González ¹ , María Isabel Sánchez-Reus ² , Eduardo Madrigal-Santillán ¹ , Carmen Valadez-Vega ¹ , Tomas Fregoso-Aguilar ^3,* , Jorge Alberto Mendoza-Pérez ³ , José Gutiérrez-Salinas ⁴  and Jaime Esquivel-Soto ⁵

¹ Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla, 42080 Pachuca de Soto, Hgo, Mexico ² Instituto de Bioquímica (CSIC-UCM), Facultad de Farmacia, Ciudad Universitaria, Plaza de Ramón y Cajal S/N, 28040 Madrid, Spain ³ Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., 07700, Mexico ⁴ Laboratorio de Bioquímica y Medicina Experimental, División de Investigación Biomédica, Centro Médico Nacional “20 de Noviembre”, ISSSTE, México, D.F., 03229, Mexico ⁵ Facultad de Odontologia, Circuito Escolar S/N, Ciudad Universitaria (UNAM), México, D.F., 04510, Mexico

* Authors to whom correspondence should be addressed.

Received: 4 August 2011; in revised form: 11 September 2011 / Accepted: 19 September 2011 / Published: 29 September 2011

(This article belongs to the Section Molecular Diversity)

Download PDF Full-Text [842 KB, uploaded 29 September 2011 14:10 CEST]

Abstract: It is well known that gadolinium chloride (GD) attenuates drug-induced hepatotoxicity by selectively inactivating Kupffer cells. In the present study the effect of GD in reference to cell cycle and postnecrotic liver regeneration induced by thioacetamide (TA) in rats was studied. Two months male rats, intraveously pretreated with a single dose of GD (0.1 mmol/Kg), were intraperitoneally injected with TA (6.6 mmol/Kg). Samples of blood and liver were obtained from rats at 0, 12, 24, 48, 72 and 96 h following TA intoxication. Parameters related to liver damage were determined in blood. In order to evaluate the mechanisms involved in the post-necrotic regenerative state, the levels of cyclin D and cyclin E as well as protein p27 and Proliferating Cell Nuclear Antigen (PCNA) were determined in liver extracts because of their roles in the control of cell cycle check-points. The results showed that GD significantly reduced the extent of necrosis. Noticeable changes were detected in the levels of cyclin D1, cyclin E, p27 and PCNA when compared to those induced by thioacetamide. Thus GD pre-treatment reduced TA-induced liver injury and accelerated the postnecrotic liver regeneration. These results demonstrate that Kupffer cells are involved in TA-induced liver and also in the postnecrotic proliferative liver states.

Keywords: gadolinium chloride; kupffer cells; thioacetamide hepatotoxicity; cell cycle; cyclins

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