Molecules 2011, 16(10), 8305-8318; doi:10.3390/molecules16108305
Article

Inhibitory Effects of Constituents from Euphorbia lunulata on Differentiation of 3T3-L1 Cells and Nitric Oxide Production in RAW264.7 Cells

Zhi-Gang Yang 1,† email, Liu-Nan Jia 1,† email, Yan Shen 2 email, Atsuko Ohmura 3 email and Susumu Kitanaka 1,* email
1 School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba 274-8555, Japan 2 College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan 3 Saitama Prefectural Pharmaceutical Affairs Divison, 3-15-1 Takasago, Saitama, Saitama 330-9301, Japan † These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 9 August 2011; in revised form: 14 September 2011 / Accepted: 27 September 2011 / Published: 29 September 2011
(This article belongs to the Section Natural Products)
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Abstract: A new flavonol galactopyranoside, myricetin 3-O-(2'',3''-digalloyl)-β-D-galactopyranoide (1), and 23 known constituents, including myricetin 3-O-(2''-galloyl)-β-D-galactopyranoide (2), myricitrin (3), myricetin (4), quercetin 3-O-(2'', 3''-digalloyl)-β-D-galactopyranoide (5), quercetin 3-O-(2''-galloyl)-β-D-galactopyranoide (6), hyperin (7), isoquercetrin (8), quercetin (9), kaempferol (10), apigenin (11), luteolin (12), 3-O-methylquercetin (13), 5,7,2',5'-tetrahydroxyflavone (14), 1,3,4,6-tetra-O-galloyl-β-D-glucose (15), 1,2,6-tri-O-galloyl-β-D-glucose (16), 1,3,6-tri-O-galloyl-β-D-glucose (17), gallic acid (18), protocatechuic acid (19), 3,4,5-trimethoxybenzoic acid (20), 2,6-dihydroxyacetophenone (21), 3,3'-di-O-methylellagic acid (22), ellagic acid (23) and esculetin (24) were isolated from Euphorbia lunulata Bge. Their structures were determined by spectroscopic analysis. Isolated hydrolysable tannins, flavonoids, and flavonol galactopyranoside gallates showed significant inhibition of the differentiation of 3T3-L1 preadipocytes and triglyceride accumulation in maturing adipocytes, and nitric oxide production in RAW 264.7 cells.
Keywords: Euphorbia lunulata Bge.; flavonol galactopyranoside gallate; phenolic constituents; adipocyte; macrophage

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MDPI and ACS Style

Yang, Z.-G.; Jia, L.-N.; Shen, Y.; Ohmura, A.; Kitanaka, S. Inhibitory Effects of Constituents from Euphorbia lunulata on Differentiation of 3T3-L1 Cells and Nitric Oxide Production in RAW264.7 Cells. Molecules 2011, 16, 8305-8318.

AMA Style

Yang Z-G, Jia L-N, Shen Y, Ohmura A, Kitanaka S. Inhibitory Effects of Constituents from Euphorbia lunulata on Differentiation of 3T3-L1 Cells and Nitric Oxide Production in RAW264.7 Cells. Molecules. 2011; 16(10):8305-8318.

Chicago/Turabian Style

Yang, Zhi-Gang; Jia, Liu-Nan; Shen, Yan; Ohmura, Atsuko; Kitanaka, Susumu. 2011. "Inhibitory Effects of Constituents from Euphorbia lunulata on Differentiation of 3T3-L1 Cells and Nitric Oxide Production in RAW264.7 Cells." Molecules 16, no. 10: 8305-8318.

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