Molecules 2011, 16(1), 847-856; doi:10.3390/molecules16010847
Article

Enhancement of Diepoxin ζ Production by Yeast Extract and Its Fractions in Liquid Culture of Berkleasmium-Like Endophytic Fungus Dzf12 from Dioscorea zingiberensis

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Received: 14 December 2010; in revised form: 7 January 2011 / Accepted: 18 January 2011 / Published: 19 January 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: This study was to examine the effects of yeast extract (YE) and its fractions (YE1 and YE2) on the growth and diepoxin ζ (a spirobisnaphthalene with a diversity of bioactivities) production in liquid culture of Berkleasmium-like endophytic fungus Dzf12 from Dioscorea zingiberensis. When YE was applied to the liquid medium at 10 g/L on day 3 of culture, the diepoxin ζ production was most effectively enhanced 3.2-fold (378.70 mg/L versus 120.09 mg/L in control) after another 10 days culture. Feeding with 15 g/L of YE on day 9, the mycelia biomass reached 16.44 g/L, about 2.3-fold in comparison with the control (7.15 g/L). The polysaccharide fraction (YE1) was mainly responsible for stimulating diepoxin ζ accumulation, and the non-polysaccharide fraction (YE2) was mainly responsible for promoting mycelia growth. The results showed that the diepoxin ζ production in liquid culture of endophyte Dzf12 could be effectively enhanced by YE and its fractions.
Keywords: diepoxin ζ; Berkleasmium-like endophytic fungus Dzf12; Dioscorea zingiberensis; yeast extract (YE); polysaccharide fraction; non-polysaccharide fraction; enhancement
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MDPI and ACS Style

Zhao, J.; Zheng, B.; Li, Y.; Shan, T.; Mou, Y.; Lu, S.; Li, P.; Zhou, L. Enhancement of Diepoxin ζ Production by Yeast Extract and Its Fractions in Liquid Culture of Berkleasmium-Like Endophytic Fungus Dzf12 from Dioscorea zingiberensis. Molecules 2011, 16, 847-856.

AMA Style

Zhao J, Zheng B, Li Y, Shan T, Mou Y, Lu S, Li P, Zhou L. Enhancement of Diepoxin ζ Production by Yeast Extract and Its Fractions in Liquid Culture of Berkleasmium-Like Endophytic Fungus Dzf12 from Dioscorea zingiberensis. Molecules. 2011; 16(1):847-856.

Chicago/Turabian Style

Zhao, Jianglin; Zheng, Bingbing; Li, Yan; Shan, Tijiang; Mou, Yan; Lu, Shqiong; Li, Peiqin; Zhou, Ligang. 2011. "Enhancement of Diepoxin ζ Production by Yeast Extract and Its Fractions in Liquid Culture of Berkleasmium-Like Endophytic Fungus Dzf12 from Dioscorea zingiberensis." Molecules 16, no. 1: 847-856.

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