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Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy
Technische Universität Braunschweig, Institute of Biochemistry and Biotechnology, Department of Biotechnology, Spielmannstr. 7, 38106 Braunschweig, Germany
Present address: Merck KGaA, Frankfurter Str. 250, 64293 Darmstadt, Germany
* Author to whom correspondence should be addressed.
Received: 10 November 2010; in revised form: 4 January 2011 / Accepted: 7 January 2011 / Published: 10 January 2011
Abstract: Twenty years after its development, antibody phage display using filamentous bacteriophage represents the most successful in vitro antibody selection technology. Initially, its development was encouraged by the unique possibility of directly generating recombinant human antibodies for therapy. Today, antibody phage display has been developed as a robust technology offering great potential for automation. Generation of monospecific binders provides a valuable tool for proteome research, leading to highly enhanced throughput and reduced costs. This review presents the phage display technology, application areas of antibodies in research, diagnostics and therapy and the use of antibody phage display for these applications.
Keywords: antibody; scFv; phage display; cancer; diagnostics; proteome research; panning
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Schirrmann, T.; Meyer, T.; Schütte, M.; Frenzel, A.; Hust, M. Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy. Molecules 2011, 16, 412-426.
Schirrmann T, Meyer T, Schütte M, Frenzel A, Hust M. Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy. Molecules. 2011; 16(1):412-426.
Schirrmann, Thomas; Meyer, Torsten; Schütte, Mark; Frenzel, André; Hust, Michael. 2011. "Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy." Molecules 16, no. 1: 412-426.