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Content of Phenolic Compounds and Antioxidant Capacity in Fruits of Apricot Genotypes
Department of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel University in Brno, Valticka 337, CZ-691 44 Lednice, Czech Republic
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Kolejni 4, CZ-612 00 Brno, Czech Republic
Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1-3, CZ-612 42 Brno, Czech Republic
Department of Fruit Growing, Faculty of Horticulture, Mendel University in Brno, Valticka 337, CZ-691 44 Lednice, Czech Republic
University Institute, Tomas Bata University in Zlin, T. G. Masaryka 275, CZ-762 72 Zlin, Czech Republic
* Author to whom correspondence should be addressed.
Received: 29 July 2010; in revised form: 25 August 2010 / Accepted: 1 September 2010 / Published: 7 September 2010
Abstract: Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH• (1,1-diphenyl-2-picryl-hydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (–)- and (+)- catechin.
Keywords: apricot; DPPH; TEAC; FRAP; high performance liquid chromatography (HPLC); electrochemical and spectrometric detection; Plum pox virus (PPV); dendrogram
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Sochor, J.; Zitka, O.; Skutkova, H.; Pavlik, D.; Babula, P.; Krska, B.; Horna, A.; Adam, V.; Provaznik, I.; Kizek, R. Content of Phenolic Compounds and Antioxidant Capacity in Fruits of Apricot Genotypes. Molecules 2010, 15, 6285-6305.
Sochor J, Zitka O, Skutkova H, Pavlik D, Babula P, Krska B, Horna A, Adam V, Provaznik I, Kizek R. Content of Phenolic Compounds and Antioxidant Capacity in Fruits of Apricot Genotypes. Molecules. 2010; 15(9):6285-6305.
Sochor, Jiri; Zitka, Ondrej; Skutkova, Helena; Pavlik, Dusan; Babula, Petr; Krska, Boris; Horna, Ales; Adam, Vojtech; Provaznik, Ivo; Kizek, Rene. 2010. "Content of Phenolic Compounds and Antioxidant Capacity in Fruits of Apricot Genotypes." Molecules 15, no. 9: 6285-6305.