Molecules 2010, 15(9), 6140-6151; doi:10.3390/molecules15096140
Communication

Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation

1 Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil 2 Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, 14404-600, Brazil 3 Instituto de Ciências Exatas e da Terra, Universidade Federal de Mato Grosso, MT, 78060-900, Brazil 4 Centro de Ciências Exatas e de Tecnologia, Universidade Federal de São Carlos, SP, 13565-905, Brazil
* Author to whom correspondence should be addressed.
Received: 11 August 2010; in revised form: 30 August 2010 / Accepted: 30 August 2010 / Published: 1 September 2010
(This article belongs to the Section Natural Products)
PDF Full-text Download PDF Full-Text [115 KB, uploaded 1 September 2010 14:48 CEST]
Abstract: The goal of the study was to evaluate the ability of filamentous fungi to biotransform the pentacyclic triterpene lupeol. The microbial transformations were carried out in shake flasks in different media. Experiments were also run with control flasks. Samples of each culture were taken every 24 hours, extracted with ethyl acetate, and analyzed by GC-MS. The biotransformation of lupeol by Aspergillus ochraceus and Mucor rouxii afforded two compounds in each culture, which were detected in the cultures developed for more than seven days only in the Koch’s K1 medium. The obtained data demonstrated that A. ochraceus is a good biocatalyst to introduce double bonds in the lupeol structure, whereas M. rouxii exhibits ability to biocatalyze oxygen insertions in that pentacyclic triterpene. Mass spectrometry was demonstrated to be an efficient analytical method to select promising biocatalysts for the compound investigated in this study. The biotransformation processes were influenced by the culture medium and incubation period. The obtained results open the perspective of using A. ochraceus and M. rouxii in pentacyclic triterpene biotransformations.
Keywords: biocatalysts; biotransformation; filamentous fungi; lupeol; mass spectrometry

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Carvalho, T.C.; Polizeli, A.M.; Turatti, I.C.C.; Severiano, M.E.; Carvalho, C.E.; Ambrósio, S.R.; Crotti, A.E.M.; Figueiredo, U.S.; Vieira, P.C.; Furtado, N.A.J.C. Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation. Molecules 2010, 15, 6140-6151.

AMA Style

Carvalho TC, Polizeli AM, Turatti ICC, Severiano ME, Carvalho CE, Ambrósio SR, Crotti AEM, Figueiredo US, Vieira PC, Furtado NAJC. Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation. Molecules. 2010; 15(9):6140-6151.

Chicago/Turabian Style

Carvalho, Tatiane C. de; Polizeli, Aline M.; Turatti, Izabel C. C.; Severiano, Marcela E.; Carvalho, Carlos E. de; Ambrósio, Sérgio R.; Crotti, Antônio E. M.; Figueiredo, Uir S. de; Vieira, Paulo C.; Furtado, Niege A. J. C. 2010. "Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation." Molecules 15, no. 9: 6140-6151.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert