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Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil
Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, 14404-600, Brazil
Instituto de Ciências Exatas e da Terra, Universidade Federal de Mato Grosso, MT, 78060-900, Brazil
Centro de Ciências Exatas e de Tecnologia, Universidade Federal de São Carlos, SP, 13565-905, Brazil
* Author to whom correspondence should be addressed.
Received: 11 August 2010; in revised form: 30 August 2010 / Accepted: 30 August 2010 / Published: 1 September 2010
Abstract: The goal of the study was to evaluate the ability of filamentous fungi to biotransform the pentacyclic triterpene lupeol. The microbial transformations were carried out in shake flasks in different media. Experiments were also run with control flasks. Samples of each culture were taken every 24 hours, extracted with ethyl acetate, and analyzed by GC-MS. The biotransformation of lupeol by Aspergillus ochraceus and Mucor rouxii afforded two compounds in each culture, which were detected in the cultures developed for more than seven days only in the Koch’s K1 medium. The obtained data demonstrated that A. ochraceus is a good biocatalyst to introduce double bonds in the lupeol structure, whereas M. rouxii exhibits ability to biocatalyze oxygen insertions in that pentacyclic triterpene. Mass spectrometry was demonstrated to be an efficient analytical method to select promising biocatalysts for the compound investigated in this study. The biotransformation processes were influenced by the culture medium and incubation period. The obtained results open the perspective of using A. ochraceus and M. rouxii in pentacyclic triterpene biotransformations.
Keywords: biocatalysts; biotransformation; filamentous fungi; lupeol; mass spectrometry
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Carvalho, T.C.; Polizeli, A.M.; Turatti, I.C.C.; Severiano, M.E.; Carvalho, C.E.; Ambrósio, S.R.; Crotti, A.E.M.; Figueiredo, U.S.; Vieira, P.C.; Furtado, N.A.J.C. Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation. Molecules 2010, 15, 6140-6151.
Carvalho TC, Polizeli AM, Turatti ICC, Severiano ME, Carvalho CE, Ambrósio SR, Crotti AEM, Figueiredo US, Vieira PC, Furtado NAJC. Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation. Molecules. 2010; 15(9):6140-6151.
Carvalho, Tatiane C. de; Polizeli, Aline M.; Turatti, Izabel C. C.; Severiano, Marcela E.; Carvalho, Carlos E. de; Ambrósio, Sérgio R.; Crotti, Antônio E. M.; Figueiredo, Uir S. de; Vieira, Paulo C.; Furtado, Niege A. J. C. 2010. "Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation." Molecules 15, no. 9: 6140-6151.