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Molecules 2010, 15(9), 6152-6167; doi:10.3390/molecules15096152
Article

Antioxidant Capacity of Pure Compounds and Complex Mixtures Evaluated by the ORAC-Pyrogallol Red Assay in the Presence of Triton X-100 Micelles

1
, 2
, 1
, 1
, 3
 and 1,*
1 Departamento de Farmacia, Facultad de Química, Pontificia Universidad Católica de Chile, C.P. 782 0436, Santiago, Chile 2 Escuela de Química, Universidad Nacional de Colombia, Sede Medellin, AA 1027 Medellin, Colombia 3 Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile
* Author to whom correspondence should be addressed.
Received: 22 July 2010 / Revised: 18 August 2010 / Accepted: 27 August 2010 / Published: 1 September 2010
(This article belongs to the Special Issue Antioxidants)
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Abstract

The protective effect of different antioxidants and complex mixtures on the consumption of pyrogallol red (PGR) induced by peroxyl radicals was studied in the absence and presence of Triton X-100 micelles. The presence of micelles decreased significantly the protection of PGR afforded by lipophilic antioxidants (β-carotene, octyl gallate), while no effect of micelles was observed for hydrophilic antioxidants such as Trolox, caffeic acid, gallic acid, and ascorbic acid. In the presence of complex mixtures a clear effect of Triton X-100 micelles was also observed in the protection afforded by wines, tea infusions, and seed extracts of Eugenia jambolana and Myrciaria cauliflora. On the other hand, no effect of micelles was observed for orange juice and pulp fruit extracts. The ORAC (Oxygen Radical Absorbance Capacity) index was evaluated in the absence (ORAC-PGR) and presence of Triton X-100 micelles (ORAC-PGRMIC). Triton X-100 micelles affect ORAC-PGR values of antioxidants in a lipophilicity-dependent way. From the obtained results, we conclude that ORAC-PGR and ORAC-PGRMIC assays could be considered as an alternative to estimate the antioxidant ability (ORAC-PGR) and to infer the association to Triton X-100 micelles (ORAC-PGR/ORAC-PGRMIC) of pure antioxidants and their complex mixtures.
Keywords: ORAC; pyrogallol red; micelles; Triton X-100; antioxidants ORAC; pyrogallol red; micelles; Triton X-100; antioxidants
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Romero, M.; Rojano, B.; Mella-Raipán, J.; Pessoa-Mahana, C.D.; Lissi, E.; López-Alarcón, C. Antioxidant Capacity of Pure Compounds and Complex Mixtures Evaluated by the ORAC-Pyrogallol Red Assay in the Presence of Triton X-100 Micelles. Molecules 2010, 15, 6152-6167.

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