Next Article in Journal
Previous Article in Journal
Molecules 2010, 15(6), 4478-4489; doi:10.3390/molecules15064478
Article

In Vitro Antioxidant Activity and Hepatoprotective Effects of Lentinula edodes against Paracetamol-Induced Hepatotoxicity

1, 2, 3, 2, 4 and 1,5,*
Received: 6 May 2010; in revised form: 9 June 2010 / Accepted: 10 June 2010 / Published: 23 June 2010
Download PDF [524 KB, updated 18 June 2014; original version uploaded 18 June 2014]
Abstract: Background: The objective of this study was to investigate the antioxidant and hepatoprotective effects of methanolic extracts of L. edodes and the determination of their total phenolics content. Results: The amount of total phenolics was estimated to be 70.83 mg Gallic Acid Equivalent (GAE) per gram of dry extract. The antioxidant activity of the L. edodes extract was 39.0% at a concentration of 1 mg/mL and was also concentration dependant, with an EC50 value of 4.4 mg/mL. Different groups of animals (Wister albino mice) were administered paracetamol (1 g/kg, p.o.). L. edodes extract at a dose of 200 mg/kg was administered to the paracetamol treated mice for seven days. The effects of L. edodes extract on serum transaminases (SGOT, SGPT), alkaline phosphatase (ALP) and bilirubin were measured in the paracetamol-induced hepatotoxic mice. L. edodes extract produced significant (p < 0.05) hepatoprotective effects by decreasing the activity of serum enzymes and bilirubin. Conclusions: From these results, it was suggested that L. edodes extract could perhaps protect liver cells from paracetamol-induced liver damage by its antioxidative effect on hepatocytes, hence diminishing or eliminating the harmful effects of toxic metabolites of paracetamol.
Keywords: Lentinula edodes; hepatoprotective activity; antioxidant activity; liver marker enzymes Lentinula edodes; hepatoprotective activity; antioxidant activity; liver marker enzymes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Sasidharan, S.; Aravindran, S.; Latha, L.Y.; Vijenthi, R.; Saravanan, D.; Amutha, S. In Vitro Antioxidant Activity and Hepatoprotective Effects of Lentinula edodes against Paracetamol-Induced Hepatotoxicity. Molecules 2010, 15, 4478-4489.

AMA Style

Sasidharan S, Aravindran S, Latha LY, Vijenthi R, Saravanan D, Amutha S. In Vitro Antioxidant Activity and Hepatoprotective Effects of Lentinula edodes against Paracetamol-Induced Hepatotoxicity. Molecules. 2010; 15(6):4478-4489.

Chicago/Turabian Style

Sasidharan, Sreenivasan; Aravindran, Sugumaran; Latha, Lachimanan Yoga; Vijenthi, Ratnasamy; Saravanan, Dharmaraj; Amutha, Santhanam. 2010. "In Vitro Antioxidant Activity and Hepatoprotective Effects of Lentinula edodes against Paracetamol-Induced Hepatotoxicity." Molecules 15, no. 6: 4478-4489.



Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert