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In Vitro Antioxidant Activity and Hepatoprotective Effects of Lentinula edodes against Paracetamol-Induced Hepatotoxicity
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Jalan Bedong-Semeling, Batu 3 ½, Bukit Air Nasi, Bedong 08100, Kedah, Malaysia
School of Biological Sciences, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
Centre for Drug Research, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar, Madurai, India
* Author to whom correspondence should be addressed.
Received: 6 May 2010; in revised form: 9 June 2010 / Accepted: 10 June 2010 / Published: 23 June 2010
Abstract: Background: The objective of this study was to investigate the antioxidant and hepatoprotective effects of methanolic extracts of L. edodes and the determination of their total phenolics content. Results: The amount of total phenolics was estimated to be 70.83 mg Gallic Acid Equivalent (GAE) per gram of dry extract. The antioxidant activity of the L. edodes extract was 39.0% at a concentration of 1 mg/mL and was also concentration dependant, with an EC50 value of 4.4 mg/mL. Different groups of animals (Wister albino mice) were administered paracetamol (1 g/kg, p.o.). L. edodes extract at a dose of 200 mg/kg was administered to the paracetamol treated mice for seven days. The effects of L. edodes extract on serum transaminases (SGOT, SGPT), alkaline phosphatase (ALP) and bilirubin were measured in the paracetamol-induced hepatotoxic mice. L. edodes extract produced significant (p < 0.05) hepatoprotective effects by decreasing the activity of serum enzymes and bilirubin. Conclusions: From these results, it was suggested that L. edodes extract could perhaps protect liver cells from paracetamol-induced liver damage by its antioxidative effect on hepatocytes, hence diminishing or eliminating the harmful effects of toxic metabolites of paracetamol.
Keywords: Lentinula edodes; hepatoprotective activity; antioxidant activity; liver marker enzymes
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Sasidharan, S.; Aravindran, S.; Latha, L.Y.; Vijenthi, R.; Saravanan, D.; Amutha, S. In Vitro Antioxidant Activity and Hepatoprotective Effects of Lentinula edodes against Paracetamol-Induced Hepatotoxicity. Molecules 2010, 15, 4478-4489.
Sasidharan S, Aravindran S, Latha LY, Vijenthi R, Saravanan D, Amutha S. In Vitro Antioxidant Activity and Hepatoprotective Effects of Lentinula edodes against Paracetamol-Induced Hepatotoxicity. Molecules. 2010; 15(6):4478-4489.
Sasidharan, Sreenivasan; Aravindran, Sugumaran; Latha, Lachimanan Yoga; Vijenthi, Ratnasamy; Saravanan, Dharmaraj; Amutha, Santhanam. 2010. "In Vitro Antioxidant Activity and Hepatoprotective Effects of Lentinula edodes against Paracetamol-Induced Hepatotoxicity." Molecules 15, no. 6: 4478-4489.