Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Molecules 2010, 15(5), 3744-3756; doi:10.3390/molecules15053744
Article

Oak (Quercus frainetto Ten.) Honeydew Honey—Approach to Screening of Volatile Organic Composition and Antioxidant Capacity (DPPH and FRAP Assay)

1,*  and 2
Received: 26 April 2010; in revised form: 17 May 2010 / Accepted: 24 May 2010 / Published: 25 May 2010
(This article belongs to the Special Issue Antioxidants)
Download PDF [199 KB, uploaded 18 June 2014]
Abstract: Two samples of oak honeydew honey were investigated. Headspace solid-phase microextraction (HS-SPME) combined with GC and GC/MS enabled identification of the most volatile organic headspace compounds being dominated by terpenes(mainly cis- and trans-linalool oxides). The volatile and less-volatile organic composition of the samples was obtained by ultrasonic assisted extraction (USE) with two solvents (1:2 (v/v) pentane -diethyl ether mixture and dichloromethane) followed by GC and GC/MS analysis. Shikimic pathway derivatives are of particular interest with respect to the botanical origin of honey and the most abundant was phenylacetic acid (up to 16.4%). Antiradical activity (DPPH assay) of the honeydew samples was 4.5 and 5.1 mmol TEAC/kg. Ultrasonic solvent extracts showed several dozen times higher antiradical capacity in comparison to the honeydew. Antioxidant capacity (FRAP assay) of honeydew samples was 4.8 and 16.1 mmol Fe2+/kg, while the solvent mixture extracts showed antioxidant activity of 374.5 and 955.9 Fe2+/kg, respectively, and the dichloromethane extracts 127.3 and 101.5 mmol Fe2+/kg.
Keywords: Quercus frainetto Ten. honeydew honey; headspace solid-phase microextraction (HS-SPME); ultrasonic solvent extraction (USE); gas chromatography and mass spectrometry (GC and GC/MS); DPPH and FRAP assay Quercus frainetto Ten. honeydew honey; headspace solid-phase microextraction (HS-SPME); ultrasonic solvent extraction (USE); gas chromatography and mass spectrometry (GC and GC/MS); DPPH and FRAP assay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Jerković, I.; Marijanović, Z. Oak (Quercus frainetto Ten.) Honeydew Honey—Approach to Screening of Volatile Organic Composition and Antioxidant Capacity (DPPH and FRAP Assay). Molecules 2010, 15, 3744-3756.

AMA Style

Jerković I, Marijanović Z. Oak (Quercus frainetto Ten.) Honeydew Honey—Approach to Screening of Volatile Organic Composition and Antioxidant Capacity (DPPH and FRAP Assay). Molecules. 2010; 15(5):3744-3756.

Chicago/Turabian Style

Jerković, Igor; Marijanović, Zvonimir. 2010. "Oak (Quercus frainetto Ten.) Honeydew Honey—Approach to Screening of Volatile Organic Composition and Antioxidant Capacity (DPPH and FRAP Assay)." Molecules 15, no. 5: 3744-3756.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert