Molecules 2010, 15(5), 3744-3756; doi:10.3390/molecules15053744
Article

Oak (Quercus frainetto Ten.) Honeydew Honey—Approach to Screening of Volatile Organic Composition and Antioxidant Capacity (DPPH and FRAP Assay)

1 Faculty of Chemistry and Technology, University of Split, N. Tesle 10/V, 21000 Split, Croatia 2 Marko Marulić Polytechnic in Knin, P. Krešimira IV 30, 22300 Knin, Croatia
* Author to whom correspondence should be addressed.
Received: 26 April 2010; in revised form: 17 May 2010 / Accepted: 24 May 2010 / Published: 25 May 2010
(This article belongs to the Special Issue Antioxidants)
PDF Full-text Download PDF Full-Text [199 KB, uploaded 25 May 2010 16:39 CEST]
Abstract: Two samples of oak honeydew honey were investigated. Headspace solid-phase microextraction (HS-SPME) combined with GC and GC/MS enabled identification of the most volatile organic headspace compounds being dominated by terpenes(mainly cis- and trans-linalool oxides). The volatile and less-volatile organic composition of the samples was obtained by ultrasonic assisted extraction (USE) with two solvents (1:2 (v/v) pentane -diethyl ether mixture and dichloromethane) followed by GC and GC/MS analysis. Shikimic pathway derivatives are of particular interest with respect to the botanical origin of honey and the most abundant was phenylacetic acid (up to 16.4%). Antiradical activity (DPPH assay) of the honeydew samples was 4.5 and 5.1 mmol TEAC/kg. Ultrasonic solvent extracts showed several dozen times higher antiradical capacity in comparison to the honeydew. Antioxidant capacity (FRAP assay) of honeydew samples was 4.8 and 16.1 mmol Fe2+/kg, while the solvent mixture extracts showed antioxidant activity of 374.5 and 955.9 Fe2+/kg, respectively, and the dichloromethane extracts 127.3 and 101.5 mmol Fe2+/kg.
Keywords: Quercus frainetto Ten. honeydew honey; headspace solid-phase microextraction (HS-SPME); ultrasonic solvent extraction (USE); gas chromatography and mass spectrometry (GC and GC/MS); DPPH and FRAP assay

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Jerković, I.; Marijanović, Z. Oak (Quercus frainetto Ten.) Honeydew Honey—Approach to Screening of Volatile Organic Composition and Antioxidant Capacity (DPPH and FRAP Assay). Molecules 2010, 15, 3744-3756.

AMA Style

Jerković I, Marijanović Z. Oak (Quercus frainetto Ten.) Honeydew Honey—Approach to Screening of Volatile Organic Composition and Antioxidant Capacity (DPPH and FRAP Assay). Molecules. 2010; 15(5):3744-3756.

Chicago/Turabian Style

Jerković, Igor; Marijanović, Zvonimir. 2010. "Oak (Quercus frainetto Ten.) Honeydew Honey—Approach to Screening of Volatile Organic Composition and Antioxidant Capacity (DPPH and FRAP Assay)." Molecules 15, no. 5: 3744-3756.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert