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Molecules 2010, 15(5), 3694-3708; doi:10.3390/molecules15053694
Article

Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice

1,2
, 3
, 1
, 4
, 1
 and 1,*
1 College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China 2 Infinitus (China) Co., Ltd., Jiangmen 529156, China 3 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China 4 Guangzhou University of Traditional Chinese Medicine, Guangzhou 510632, China
* Author to whom correspondence should be addressed.
Received: 30 March 2010 / Revised: 12 May 2010 / Accepted: 14 May 2010 / Published: 25 May 2010
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Abstract

Response surface methodology was employed to optimize the conditions for alkaline extraction of polysaccharides from Ganoderma lucidum. The results indicated that the optimum conditions were an extraction temperature of 60.1 ºC, an extraction time of 77.3 min, a sodium hydroxide (NaOH) concentration of 5.1% and a substrate/liquid ratio of 1:21.4. Immunological assays results have shown that the alkaline soluble polysaccharides have no noticeable effects on monocyte phagocytosis and immune organ (spleen, thymus) weight of of immunocompromised mice at the tested dosages. However, they could restore delayed type hypersensitivity reaction to dinitrofluorobenzene (DFNB), hemolysis antibody levels at the three doses applied, and improve the natural killer cell activity at the high-dose and medium dose.
Keywords: Ganoderma lucidum; alkaline extraction; polysaccharides; yield; immune activity Ganoderma lucidum; alkaline extraction; polysaccharides; yield; immune activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huang, S.-Q.; Li, J.-W.; Wang, Z.; Pan, H.-X.; Chen, J.-X.; Ning, Z.-X. Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice. Molecules 2010, 15, 3694-3708.

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