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Determination of Essential Oil Composition from Osmanthus fragrans Tea by GC-MS Combined with a Chemometric Resolution Method
Pharmaceutical School of Xianning University, Xianning 437100, China
Research Center of Modernization of Chinese Herbal Medicines, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
Xinjiang Institute for Food and Drug Control, Urumqi 830002, China
* Author to whom correspondence should be addressed.
Received: 11 February 2010; in revised form: 11 March 2010 / Accepted: 21 April 2010 / Published: 19 May 2010
Abstract: The essential oils of the Osmanthus fragrans tea (OFT), Osmanthus fragrans (OF) and green tea (GT) had been extracted by steam distillation and analyzed by gas chromatography-mass spectrometry (GC-MS) with the help of heuristic evolving latent projections (HELP), an effective chemometric resolution method (CRM). The overlapping peak clusters were resolved into pure chromatograms and pure mass spectra with HELP. The qualitative analysis was performed by similarity searches in the National Institute of Standards and Technology (NIST) mass spectra database with the obtained pure mass spectrum of each component. Identification of some compounds was also assisted by comparison of temperature-programmed retention indices (PTRI) with authentic standards included in our own laboratory database under construction. The quantitative results were obtained by overall volume integration (OVI) method. A total of 67, 73 and 53 components in essential oils of the OFT, OF and GT were identified, accounting for 90.83%, 93.65% and 89.97% total contents of the essential oil of OFT, OF and GT, respectively.
Keywords: Osmanthus fragrans tea; essential oil; GC-MS; chemometric resolution method; temperature-programmed retention indices
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MDPI and ACS Style
Hu, C.-D.; Liang, Y.-Z.; Guo, F.-Q.; Li, X.-R.; Wang, W.-P. Determination of Essential Oil Composition from Osmanthus fragrans Tea by GC-MS Combined with a Chemometric Resolution Method. Molecules 2010, 15, 3683-3693.
Hu C-D, Liang Y-Z, Guo F-Q, Li X-R, Wang W-P. Determination of Essential Oil Composition from Osmanthus fragrans Tea by GC-MS Combined with a Chemometric Resolution Method. Molecules. 2010; 15(5):3683-3693.
Hu, Chun-Di; Liang, Yi-Zeng; Guo, Fang-Qiu; Li, Xiao-Ru; Wang, Wei-Ping. 2010. "Determination of Essential Oil Composition from Osmanthus fragrans Tea by GC-MS Combined with a Chemometric Resolution Method." Molecules 15, no. 5: 3683-3693.