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Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment
Polymer Centre, Faculty of Technology, Tomas Bata University in Zlín, T.G.M Sq. 275, 76272, Zlín, Czech Republic
Faculty of Sciences, Institute of Experimental Biology, Masaryk University Brno, Kotlářska 2, 61137, Brno, Czech Republic
Tomas Bata University in Zlín, T.G.M Sq. 5555, 76001, Zlín, Czech Republic
Plasma Laboratory, Department of Surface Engineering, Jožef Stefan Institute, Jamova cesta 39, SI-1000, Ljubljana, Slovenia
* Author to whom correspondence should be addressed.
Received: 22 January 2010; in revised form: 15 April 2010 / Accepted: 19 April 2010 / Published: 20 April 2010
Abstract: Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.
Keywords: plasma treatment; atelocollagen; surface modification; HaCaT; cell proliferation
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García, J.L.; Asadinezhad, A.; Pacherník, J.; Lehocký, M.; Junkar, I.; Humpolíček, P.; Sáha, P.; Valášek, P. Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment. Molecules 2010, 15, 2845-2856.
García JL, Asadinezhad A, Pacherník J, Lehocký M, Junkar I, Humpolíček P, Sáha P, Valášek P. Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment. Molecules. 2010; 15(4):2845-2856.
García, Jorge López; Asadinezhad, Ahmad; Pacherník, Jiří; Lehocký, Marián; Junkar, Ita; Humpolíček, Petr; Sáha, Petr; Valášek, Pavel. 2010. "Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment." Molecules 15, no. 4: 2845-2856.