Molecules 2010, 15(4), 2845-2856; doi:10.3390/molecules15042845
Article

Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment

Jorge López García 1 email, Ahmad Asadinezhad 1 email, Jiří Pacherník 2 email, Marián Lehocký 3,* email, Ita Junkar 4 email, Petr Humpolíček 3 email, Petr Sáha 1 email and Pavel Valášek 3 email
1 Polymer Centre, Faculty of Technology, Tomas Bata University in Zlín, T.G.M Sq. 275, 76272, Zlín, Czech Republic 2 Faculty of Sciences, Institute of Experimental Biology, Masaryk University Brno, Kotlářska 2, 61137, Brno, Czech Republic 3 Tomas Bata University in Zlín, T.G.M Sq. 5555, 76001, Zlín, Czech Republic 4 Plasma Laboratory, Department of Surface Engineering, Jožef Stefan Institute, Jamova cesta 39, SI-1000, Ljubljana, Slovenia
* Author to whom correspondence should be addressed.
Received: 22 January 2010; in revised form: 15 April 2010 / Accepted: 19 April 2010 / Published: 20 April 2010
(This article belongs to the Special Issue Solid Phase Synthesis)
PDF Full-text Download PDF Full-Text [2432 KB, uploaded 20 April 2010 10:39 CEST]
Abstract: Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.
Keywords: plasma treatment; atelocollagen; surface modification; HaCaT; cell proliferation

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

García, J.L.; Asadinezhad, A.; Pacherník, J.; Lehocký, M.; Junkar, I.; Humpolíček, P.; Sáha, P.; Valášek, P. Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment. Molecules 2010, 15, 2845-2856.

AMA Style

García JL, Asadinezhad A, Pacherník J, Lehocký M, Junkar I, Humpolíček P, Sáha P, Valášek P. Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment. Molecules. 2010; 15(4):2845-2856.

Chicago/Turabian Style

García, Jorge López; Asadinezhad, Ahmad; Pacherník, Jiří; Lehocký, Marián; Junkar, Ita; Humpolíček, Petr; Sáha, Petr; Valášek, Pavel. 2010. "Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment." Molecules 15, no. 4: 2845-2856.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert