Abstract: Both bifidobacteria and clostridia are part of the natural gut microflora and while clostridia may be responsible for severe intestinal infections, bifidobacteria are probiotic microorganisms belonging to the most important prospective bacteria in the bowel. The antimicrobial activity of biochanin A was tested in vitro against six Bifidobacterium spp., and eight Clostridium spp. using the broth microdilution method. Biochanin A showed an inhibition against all clostridia in the range of minimum inhibitory concentrations (MIC) from 64 μg/mL (for Cl. clostridioforme, strains DSM 933 and I3) to 1,024 μg/mL (for Cl. perfringens, DSM 11778). No bifidobacteria were suppressed at four-fold higher concentration (MICs > 4,096) than MIC of Cl. perfringens. These results indicate selective growth inhibition of biochanin A and its potential use in antimicrobial prevention and/or protection.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Sklenickova, O.; Flesar, J.; Kokoska, L.; Vlkova, E.; Halamova, K.; Malik, J. Selective Growth Inhibitory Effect of Biochanin A Against Intestinal Tract Colonizing Bacteria. Molecules 2010, 15, 1270-1279.
Sklenickova O, Flesar J, Kokoska L, Vlkova E, Halamova K, Malik J. Selective Growth Inhibitory Effect of Biochanin A Against Intestinal Tract Colonizing Bacteria. Molecules. 2010; 15(3):1270-1279.
Sklenickova, Olga; Flesar, Jaroslav; Kokoska, Ladislav; Vlkova, Eva; Halamova, Katerina; Malik, Jan. 2010. "Selective Growth Inhibitory Effect of Biochanin A Against Intestinal Tract Colonizing Bacteria." Molecules 15, no. 3: 1270-1279.