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Selective Growth Inhibitory Effect of Biochanin A Against Intestinal Tract Colonizing Bacteria
Department of Crop Sciences and Agroforestry, Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Kamycka 129, Prague 6, 165 21, Czech Republic
Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague 6, 165 21, Czech Republic
* Author to whom correspondence should be addressed.
Received: 13 January 2010; in revised form: 5 February 2010 / Accepted: 1 March 2010 / Published: 3 March 2010
Abstract: Both bifidobacteria and clostridia are part of the natural gut microflora and while clostridia may be responsible for severe intestinal infections, bifidobacteria are probiotic microorganisms belonging to the most important prospective bacteria in the bowel. The antimicrobial activity of biochanin A was tested in vitro against six Bifidobacterium spp., and eight Clostridium spp. using the broth microdilution method. Biochanin A showed an inhibition against all clostridia in the range of minimum inhibitory concentrations (MIC) from 64 μg/mL (for Cl. clostridioforme, strains DSM 933 and I3) to 1,024 μg/mL (for Cl. perfringens, DSM 11778). No bifidobacteria were suppressed at four-fold higher concentration (MICs > 4,096) than MIC of Cl. perfringens. These results indicate selective growth inhibition of biochanin A and its potential use in antimicrobial prevention and/or protection.
Keywords: phytoestrogen; isoflavonoid; bifidobacteria; clostridia; selective antimicrobial activity
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Sklenickova, O.; Flesar, J.; Kokoska, L.; Vlkova, E.; Halamova, K.; Malik, J. Selective Growth Inhibitory Effect of Biochanin A Against Intestinal Tract Colonizing Bacteria. Molecules 2010, 15, 1270-1279.
Sklenickova O, Flesar J, Kokoska L, Vlkova E, Halamova K, Malik J. Selective Growth Inhibitory Effect of Biochanin A Against Intestinal Tract Colonizing Bacteria. Molecules. 2010; 15(3):1270-1279.
Sklenickova, Olga; Flesar, Jaroslav; Kokoska, Ladislav; Vlkova, Eva; Halamova, Katerina; Malik, Jan. 2010. "Selective Growth Inhibitory Effect of Biochanin A Against Intestinal Tract Colonizing Bacteria." Molecules 15, no. 3: 1270-1279.