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Molecules 2010, 15(2), 780-792; doi:10.3390/molecules15020780
Article

Evaluation of Enoyl-Acyl Carrier Protein Reductase Inhibitors as Pseudomonas aeruginosa Quorum-Quenching Reagents

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Received: 7 December 2009; in revised form: 21 January 2010 / Accepted: 27 January 2010 / Published: 3 February 2010
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Abstract: Pseudomonas aeruginosa is an opportunistic pathogen which is responsible for a wide range of infections. Production of virulence factors and biofilm formation by P. aeruginosa are partly regulated by cell-to-cell communication quorum-sensing systems. Identification of quorum-quenching reagents which block the quorum-sensing process can facilitate development of novel treatment strategies for P. aeruginosa infections. We have used molecular dynamics simulation and experimental studies to elucidate the efficiencies of two potential quorum-quenching reagents, triclosan and green tea epigallocatechin gallate (EGCG), which both function as inhibitors of the enoyl-acyl carrier protein (ACP) reductase (ENR) from the bacterial type II fatty acid synthesis pathway. Our studies suggest that EGCG has a higher binding affinity towards ENR of P. aeruginosa and is an efficient quorum-quenching reagent. EGCG treatment was further shown to be able to attenuate the production of virulence factors and biofilm formation of P. aeruginosa.
Keywords: Pseudomonas aeruginosa; quorum-quenching; enoyl-acyl carrier protein reductase; molecular dynamics simulation Pseudomonas aeruginosa; quorum-quenching; enoyl-acyl carrier protein reductase; molecular dynamics simulation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yang, L.; Liu, Y.; Sternberg, C.; Molin, S. Evaluation of Enoyl-Acyl Carrier Protein Reductase Inhibitors as Pseudomonas aeruginosa Quorum-Quenching Reagents. Molecules 2010, 15, 780-792.

AMA Style

Yang L, Liu Y, Sternberg C, Molin S. Evaluation of Enoyl-Acyl Carrier Protein Reductase Inhibitors as Pseudomonas aeruginosa Quorum-Quenching Reagents. Molecules. 2010; 15(2):780-792.

Chicago/Turabian Style

Yang, Liang; Liu, Yang; Sternberg, Claus; Molin, Søren. 2010. "Evaluation of Enoyl-Acyl Carrier Protein Reductase Inhibitors as Pseudomonas aeruginosa Quorum-Quenching Reagents." Molecules 15, no. 2: 780-792.



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