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Molecules 2010, 15(2), 780-792; doi:10.3390/molecules15020780
Article

Evaluation of Enoyl-Acyl Carrier Protein Reductase Inhibitors as Pseudomonas aeruginosa Quorum-Quenching Reagents

* ,
,
 and
Department of Systems Biology, Technical University of Denmark, Kongens Lyngby, 2800, Denmark
* Author to whom correspondence should be addressed.
Received: 7 December 2009 / Revised: 21 January 2010 / Accepted: 27 January 2010 / Published: 3 February 2010
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Abstract

Pseudomonas aeruginosa is an opportunistic pathogen which is responsible for a wide range of infections. Production of virulence factors and biofilm formation by P. aeruginosa are partly regulated by cell-to-cell communication quorum-sensing systems. Identification of quorum-quenching reagents which block the quorum-sensing process can facilitate development of novel treatment strategies for P. aeruginosa infections. We have used molecular dynamics simulation and experimental studies to elucidate the efficiencies of two potential quorum-quenching reagents, triclosan and green tea epigallocatechin gallate (EGCG), which both function as inhibitors of the enoyl-acyl carrier protein (ACP) reductase (ENR) from the bacterial type II fatty acid synthesis pathway. Our studies suggest that EGCG has a higher binding affinity towards ENR of P. aeruginosa and is an efficient quorum-quenching reagent. EGCG treatment was further shown to be able to attenuate the production of virulence factors and biofilm formation of P. aeruginosa.
Keywords: Pseudomonas aeruginosa; quorum-quenching; enoyl-acyl carrier protein reductase; molecular dynamics simulation Pseudomonas aeruginosa; quorum-quenching; enoyl-acyl carrier protein reductase; molecular dynamics simulation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Yang, L.; Liu, Y.; Sternberg, C.; Molin, S. Evaluation of Enoyl-Acyl Carrier Protein Reductase Inhibitors as Pseudomonas aeruginosa Quorum-Quenching Reagents. Molecules 2010, 15, 780-792.

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