Next Article in Journal
Ancient Chinese Methods Are Remarkably Effective for the Preparation of Artemisinin-Rich Extracts of Qing Hao with Potent Antimalarial Activity
Next Article in Special Issue
Recent Applications of Ionic Liquids in Separation Technology
Previous Article in Journal
Evaluation of Enoyl-Acyl Carrier Protein Reductase Inhibitors as Pseudomonas aeruginosa Quorum-Quenching Reagents
Previous Article in Special Issue
18-Crown-6 and Dibenzo-18-crown-6 Assisted Extraction of Cesium from Water into Room Temperature Ionic Liquids and Its Correlation with Stability Constants for Cesium Complexes
Article Menu

Article Versions

Export Article

Open AccessCommunication
Molecules 2010, 15(2), 793-803; doi:10.3390/molecules15020793

The Solubility of Hen Lysozyme in Ethylammonium Nitrate/H2O Mixtures and a Novel Approach to Protein Crystallization

Department of Chemistry and Biochemistry, Arizona State University, Tempe 85287, AZ, USA
Current address: Centre for Materials and Fibre Innovation, Institute for Technology Research & Innovation, Deakin University, Geelong, Victoria 3217, Australia
*
Author to whom correspondence should be addressed.
Received: 16 December 2009 / Revised: 2 February 2010 / Accepted: 4 February 2010 / Published: 4 February 2010
(This article belongs to the Special Issue Ionic Liquids)
Download PDF [206 KB, uploaded 18 June 2014]

Abstract

We report on the solubility of hen lysozyme (HEWL) in aqueous ethylammonium nitrate (EAN) as a function of water content. We find the solubility behavior to be complex, exhibiting both a maximum (400 mg/mL) at very high EAN content) and a minimum at intermediate EAN content. We exploit this solubility profile in a novel approach to generating crystals of hydrophilic proteins, based on rehydration of a high concentration protein solution. We describe the production of crystals of X-ray diffraction quality. Two related ionic liquid solvent systems, with the same solubility profiles but different effective pH characteristics, are identified for future evaluation.
Keywords: protic ionic liquids; protein crystallization; solubility and protein stability protic ionic liquids; protein crystallization; solubility and protein stability
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Byrne, N.; Angell, C.A. The Solubility of Hen Lysozyme in Ethylammonium Nitrate/H2O Mixtures and a Novel Approach to Protein Crystallization. Molecules 2010, 15, 793-803.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top