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Molecules 2010, 15(11), 7547-7557; doi:10.3390/molecules15117547

Free Radical Scavenging Activity and Characterization of Sesquiterpenoids in Four Species of Curcuma Using a TLC Bioautography Assay and GC-MS Analysis

1
Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China
2
Institute of Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing, China
The authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 6 October 2010 / Revised: 19 October 2010 / Accepted: 25 October 2010 / Published: 27 October 2010
(This article belongs to the Special Issue Radical Chemistry)
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Abstract

The sesquiterpenoids are one of major groups of antioxidants in Curcuma besides curcuminoids. However, the real substances contributing to the antioxidant activity are still unknown. In this paper, the antioxidant activity of sesquiterpenoids in four species and two essential oils from Curcuma genus was determined and compared based on TLC separation and DPPH bioautography assay. Their antioxidant capacities were quantitatively evaluated using densitometry with detection at 530 nm (λreference = 800 nm) using vitamin C as reference. The results showed that Curcuma longa rhizomes had the highest antioxidant capacity while C. phaeocaulis presented the lowest one among the four species of Curcuma. Moreover, essential oil of C. wenyujin showed higher antioxidant potential than that of C. longa. The main TLC bands with antioxidant activity of the four species of Curcuma were collected and characterized using GC-MS, and thus curzerene, furanodiene, α-turmerone, β-turmerone and β-sesquiphellandrene were determined as major sesquiterpenoids with antioxidant activity in Curcuma.
Keywords: Curcuma; TLC; DPPH; GC-MS; Free radical scavenging activity Curcuma; TLC; DPPH; GC-MS; Free radical scavenging activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Zhao, J.; Zhang, J.-S.; Yang, B.; Lv, G.-P.; Li, S.-P. Free Radical Scavenging Activity and Characterization of Sesquiterpenoids in Four Species of Curcuma Using a TLC Bioautography Assay and GC-MS Analysis. Molecules 2010, 15, 7547-7557.

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