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The Pharmacological Effects of Morroniside and Loganin Isolated from Liuweidihuang Wan, on MC3T3-E1 Cells
National TCM Key Lab of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China
* Author to whom correspondence should be addressed.
Received: 6 September 2010; in revised form: 18 October 2010 / Accepted: 19 October 2010 / Published: 21 October 2010
Abstract: Liuweidihuang Wan (LW), initially a well-known formula for curing “wu chi wu ruan”, is commonly used nowadays for clinical treatment of Postmenopausal Osteoporosis (PO), but the identity of the effective substance(s) remains unclear. The present study was designed to evaluate the effects of morroniside and loganin isolated from LW on the proliferation, differentiation and apoptosis of MC3T3-E1 cells, as well as the possible mechanism of action. Morroniside and loganin had no effects on the proliferation of MC3T3-E1 cells, but both susbtances could improve the activity of alkaline phosphatase (ALP), and increase the contents of collagen type I and osteocalcin. Simultaneously, the mRNA expression of caspase-3, capase-9, RANKL was down-regulated and that of bcl-2 was up-regulated, which partially explains the anti-osteoporosis mechanism in MC3T3-E1 cells. In conclusion, morroniside and loganin may directly promote the differentiation and inhibit the apoptosis of MC3T3-E1 cells, and accordingly indirectly reduce bone resorption, which makes them promising natural drugs leads for treating PO in the near future.
Keywords: Liuweidihuang Wan; morroniside; loganin; osteoporosis; MC3T3-E1
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MDPI and ACS Style
Li, M.; Wang, W.; Wang, P.; Yang, K.; Sun, H.; Wang, X. The Pharmacological Effects of Morroniside and Loganin Isolated from Liuweidihuang Wan, on MC3T3-E1 Cells. Molecules 2010, 15, 7403-7414.
Li M, Wang W, Wang P, Yang K, Sun H, Wang X. The Pharmacological Effects of Morroniside and Loganin Isolated from Liuweidihuang Wan, on MC3T3-E1 Cells. Molecules. 2010; 15(10):7403-7414.
Li, Manyu; Wang, Wei; Wang, Ping; Yang, Kun; Sun, Hui; Wang, Xijun. 2010. "The Pharmacological Effects of Morroniside and Loganin Isolated from Liuweidihuang Wan, on MC3T3-E1 Cells." Molecules 15, no. 10: 7403-7414.