Next Article in Journal
Previous Article in Journal
Molecules 2010, 15(1), 432-441; doi:10.3390/molecules15010432
Article

In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs)

1, 1,* , 1, 1, 2 and 1
Received: 30 November 2009; in revised form: 2 January 2010 / Accepted: 7 January 2010 / Published: 20 January 2010
Download PDF [125 KB, uploaded 18 June 2014]
Abstract: In the present study, we investigate the effects of three different Mitragyna speciosa extracts, namely methanolic, aqueous and total alkaloid extracts, on glutathione transferase-specific activity in male Sprague Dawley rat liver cytosol in vitro and in vivo. In the in vitro study, the effect of Mitragyna speciosa extracts (0.01 to 750 µg/mL) against the specific activity of glutathione transferases was examined in rat liver cytosolic fraction from untreated rats. Our data show concentration dependent inhibition of cytosolic GSTs when Mitragyna speciosa extract was added into the reaction mixture. At the highest concentration used, the methanolic extract showed the highest GSTs specific activity inhibition (61%), followed by aqueous (50%) and total alkaloid extract (43%), respectively. In in vivo study, three different dosages; 50, 100 and 200 mg/kg for methanolic and aqueous extracts and 5, 10 and 20 mg/kg for total alkaloid extract were given orally for 14 days. An increase in GST specific activity was generally observed. However, only Mitragyna speciosa aqueous extract with a dosage of 100 mg/kg showed significant results: 129% compared to control.
Keywords: herb-drug interactions; glutathione transferases; Mitragyna speciosa; in vitro; in vivo herb-drug interactions; glutathione transferases; Mitragyna speciosa; in vitro; in vivo
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Azizi, J.; Ismail, S.; Mordi, M.N.; Ramanathan, S.; Said, M.I.M.; Mansor, S.M. In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs). Molecules 2010, 15, 432-441.

AMA Style

Azizi J, Ismail S, Mordi MN, Ramanathan S, Said MIM, Mansor SM. In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs). Molecules. 2010; 15(1):432-441.

Chicago/Turabian Style

Azizi, Juzaili; Ismail, Sabariah; Mordi, Mohd Nizam; Ramanathan, Surash; Said, Mohd Ikram Mohd; Mansor, Sharif Mahsufi. 2010. "In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs)." Molecules 15, no. 1: 432-441.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert