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Analysis of the Main Nucleosides in Cordyceps Sinensis by LC/ESI-MS
Department of Chemistry and Pharmaceutical, Quzhou College, Quzhou 324000, China
Changde Institute for Drug Control, Changde 415000, China
Biology Department, College of Science and Mathematics, California State University-Fresno, Fresno, CA 93740, USA
* Author to whom correspondence should be addressed.
Received: 10 November 2009; in revised form: 11 December 2009 / Accepted: 7 January 2010 / Published: 13 January 2010
Abstract: A sensitive, selective and reliable liquid chromatography-mass spectrometry coupled with electrospray ionization interface method for simultaneous separation and determination of thymine, adenine, adenosine and cordycepin in Cordyceps sinensis has been established. The optimum separation for these analytes was achieved using a gradient elution system and a 2.0 × 150 mm Shimadzu VP-ODS column. 2-Chloroadenosine was used as internal standard for this assay. [M+H]+ions at m/z 127, 136, 268, 252 and 302 were chosen and selective ion monitoring (SIM) mode was used for quantitative analysis of the four main nucleosides. The regression equations were linear in the range of 1.0–117.5 μg·mL-1 for thymine, 1.8-127.0 μg·mL-1 for adenine, 0.6-114.0 μg·mL-1 for adenosine and 0.5-107.5 μg·mL-1 for cordycepin. The limits of quantitation (LOQ) and detection (LOD) were 1.0 and 0.2 μg·mL-1 for thymine, 1.8 and 0.6 μg·mL-1 for adenine, 0.6 and 0.1 μg·mL-1 for adenosine and 0.5 and 0.1 μg·mL-1 for cordycepin, respectively. The recoveries of the four nucleosides ranged from 98.47 to 99.32%. The developed method was successfully used to determine nucleosides in Cordyceps sinensis from different sources.
Keywords: liquid chromatography; electrospray ionization interface; mass spectrometry; Cordyceps sinensis; nucleosides
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MDPI and ACS Style
Xie, J.-W.; Huang, L.-F.; Hu, W.; He, Y.-B.; Wong, K.P. Analysis of the Main Nucleosides in Cordyceps Sinensis by LC/ESI-MS. Molecules 2010, 15, 305-314.
Xie J-W, Huang L-F, Hu W, He Y-B, Wong KP. Analysis of the Main Nucleosides in Cordyceps Sinensis by LC/ESI-MS. Molecules. 2010; 15(1):305-314.
Xie, Jian-Wei; Huang, Lan-Fang; Hu, Wei; He, Yun-Biao; Wong, Kin Ping. 2010. "Analysis of the Main Nucleosides in Cordyceps Sinensis by LC/ESI-MS." Molecules 15, no. 1: 305-314.