Molecules 2010, 15(1), 305-314; doi:10.3390/molecules15010305

Analysis of the Main Nucleosides in Cordyceps Sinensis by LC/ESI-MS

1, 1,* email, 1, 2 and 3
Received: 10 November 2009; in revised form: 11 December 2009 / Accepted: 7 January 2010 / Published: 13 January 2010
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A sensitive, selective and reliable liquid chromatography-mass spectrometry coupled with electrospray ionization interface method for simultaneous separation and determination of thymine, adenine, adenosine and cordycepin in Cordyceps sinensis has been established. The optimum separation for these analytes was achieved using a gradient elution system and a 2.0 × 150 mm Shimadzu VP-ODS column. 2-Chloroadenosine was used as internal standard for this assay. [M+H]+ions at m/z 127, 136, 268, 252 and 302 were chosen and selective ion monitoring (SIM) mode was used for quantitative analysis of the four main nucleosides. The regression equations were linear in the range of 1.0–117.5 μg·mL-1 for thymine, 1.8-127.0 μg·mL-1 for adenine, 0.6-114.0 μg·mL-1 for adenosine and 0.5-107.5 μg·mL-1 for cordycepin. The limits of quantitation (LOQ) and detection (LOD) were 1.0 and 0.2 μg·mL-1 for thymine, 1.8 and 0.6 μg·mL-1 for adenine, 0.6 and 0.1 μg·mL-1 for adenosine and 0.5 and 0.1 μg·mL-1 for cordycepin, respectively. The recoveries of the four nucleosides ranged from 98.47 to 99.32%. The developed method was successfully used to determine nucleosides in Cordyceps sinensis from different sources.
Keywords: liquid chromatography; electrospray ionization interface; mass spectrometry; Cordyceps sinensis; nucleosides
PDF Full-text Download PDF Full-Text [163 KB, uploaded 18 June 2014 19:31 CEST]

Export to BibTeX |

MDPI and ACS Style

Xie, J.-W.; Huang, L.-F.; Hu, W.; He, Y.-B.; Wong, K.P. Analysis of the Main Nucleosides in Cordyceps Sinensis by LC/ESI-MS. Molecules 2010, 15, 305-314.

AMA Style

Xie J-W, Huang L-F, Hu W, He Y-B, Wong KP. Analysis of the Main Nucleosides in Cordyceps Sinensis by LC/ESI-MS. Molecules. 2010; 15(1):305-314.

Chicago/Turabian Style

Xie, Jian-Wei; Huang, Lan-Fang; Hu, Wei; He, Yun-Biao; Wong, Kin Ping. 2010. "Analysis of the Main Nucleosides in Cordyceps Sinensis by LC/ESI-MS." Molecules 15, no. 1: 305-314.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert