Molecules 2010, 15(1), 241-250; doi:10.3390/molecules15010241
Article

Effects of Volatile Components and Ethanolic Extract from Eclipta prostrata on Proliferation and Differentiation of Primary Osteoblasts

1 Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, China 2 Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 5 November 2009; in revised form: 16 December 2009 / Accepted: 22 December 2009 / Published: 8 January 2010
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Abstract: Eclipta prostrata, an aromatic plant, is known in Chinese herbal medicine for the treatment of various kidney diseases. In the present study, the volatile components were isolated from the aerial parts of this plant by hydrodistillation and analysed by GC–MS. A total of 55 compounds, which were the major part (91.7%) of the volatiles, were identified by matching mass spectra with a mass spectrum library (NIST 05.L). The main components were as follows: heptadecane (14.78%), 6,10,14-trimethyl-2-pentadecanone (12.80%), n-hexadecanoic acid (8.98%), pentadecane (8.68%), eudesma-4(14),11-diene (5.86%), phytol (3.77%), octadec-9-enoic acid (3.35%), 1,2-benzenedicarboxylic acid diisooctyl ester (2.74%), (Z,Z)-9,12-octadecadienoic acid (2.36%), (Z)-7,11-dimethyl-3-methylene-1,6,10-dodecatriene (2.08%) and (Z,Z,Z)-1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (2.07%). The effects of volatile components and ethanolic extract from the aerial parts of this plant on the proliferation and differentiation of primary osteoblasts were evaluated by the MTT method and measuring the activity of alkaline phosphatase (ALP activity). Both volatile components and ethanolic extract (1 μg/mL to 100 μg/mL) significantly (p < 0.01) stimulated the proliferation and increased the ALP activity of primary osteoblasts. These results propose that E. prostrata can play an important role in osteoblastic bone formation, and may possibly lead to the development of bone-forming drugs.
Keywords: Eclipta prostrata; volatile components; ethanolic extract; primary osteoblasts

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MDPI and ACS Style

Lin, X.-H.; Wu, Y.-B.; Lin, S.; Zeng, J.-W.; Zeng, P.-Y.; Wu, J.-Z. Effects of Volatile Components and Ethanolic Extract from Eclipta prostrata on Proliferation and Differentiation of Primary Osteoblasts. Molecules 2010, 15, 241-250.

AMA Style

Lin X-H, Wu Y-B, Lin S, Zeng J-W, Zeng P-Y, Wu J-Z. Effects of Volatile Components and Ethanolic Extract from Eclipta prostrata on Proliferation and Differentiation of Primary Osteoblasts. Molecules. 2010; 15(1):241-250.

Chicago/Turabian Style

Lin, Xiong-Hao; Wu, Yan-Bin; Lin, Shan; Zeng, Jian-Wei; Zeng, Pei-Yuan; Wu, Jin-Zhong. 2010. "Effects of Volatile Components and Ethanolic Extract from Eclipta prostrata on Proliferation and Differentiation of Primary Osteoblasts." Molecules 15, no. 1: 241-250.

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