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Molecules 2010, 15(1), 164-177; doi:10.3390/molecules15010164

Immunoassay Development for the Class-Specific Assay for Types I and II Pyrethroid Insecticides in Water Samples

Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
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Received: 28 October 2009 / Revised: 10 December 2009 / Accepted: 29 December 2009 / Published: 4 January 2010
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Abstract

Five generic haptens of pyrethoid insecticides, which were classified as three types, were designed and synthesized: the first (hapten 1) is for type I pyrethroids without a cyano group, the second (hapten 2 and XQ) for type II pyrethroids with a cyano group, and the third (hapten 4 and 5) for both types of pyrethroids with loss of the ester group. The hapten structures were confirmed by MS and 1H-NMR. Hapten 1 and 2 were conjugated with BSA respectively and haptens 1-5 were conjugated with OVA. Four polyclonal antisera were raised against BSA conjugates including a mixture conjugate, and twenty antibody/coating conjugate combinations were selected for studies of assay sensitivity and specificity for pyrethroids. The study revealed the best combination, which showed equal high sensitivities (I50 is around 0.02 μg mL-1) to both types of pyrethroids. The immunity results suggest that, with a mixture conjugates, a polyclonal antibody against a group of insecticides can be prepared for multi-residue assays.
Keywords: hapten; class-specific antibody; sensitivity; immunoassay; pyrethoid insecticides hapten; class-specific antibody; sensitivity; immunoassay; pyrethoid insecticides
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zhang, Q.; Zhang, W.; Wang, X.; Li, P. Immunoassay Development for the Class-Specific Assay for Types I and II Pyrethroid Insecticides in Water Samples. Molecules 2010, 15, 164-177.

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