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Molecules 2009, 14(8), 2717-2728; doi:10.3390/molecules14082717

Headspace, Volatile and Semi-Volatile Organic Compounds Diversity and Radical Scavenging Activity of Ultrasonic Solvent Extracts from Amorpha fruticosa Honey Samples

1
Faculty of Chemistry and Technology, University of Split, N. Tesle 10/V, 21000 Split, Croatia
2
Marko Marulić Polytechnic in Knin, P. Krešimira IV 30, 22300 Knin, Croatia
*
Author to whom correspondence should be addressed.
Received: 10 July 2009 / Revised: 23 July 2009 / Accepted: 27 July 2009 / Published: 27 July 2009
(This article belongs to the Special Issue Molecular Diversity Feature Papers)
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Abstract

Volatile organic compounds of Amorpha fruticosa honey samples were isolated by headspace solid-phase microextraction (HS-SPME) and ultrasonic solvent extraction (USE), followed by gas chromatography and mass spectrometry analyses (GC, GC-MS), in order to obtain complementary data for overall characterization of the honey aroma. The headspace of the honey was dominated by 2-phenylethanol (38.3–58.4%), while other major compounds were trans- and cis-linalool oxides, benzaldehyde and benzyl alcohol. 2-Phenylethanol (10.5–16.8%) and methyl syringate (5.8–8.2%) were the major compounds of ultrasonic solvent extracts, with an array of small percentages of linalool, benzene and benzoic acid derivatives, aliphatic hydrocarbons and alcohols, furan derivatives and others. The scavenging ability of the series of concentrations of the honey ultrasonic solvent extracts and the corresponding honey samples was tested by a DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Approximately 25 times lower concentration ranges (up to 2 g/L) of the extracts exhibited significantly higher free radical scavenging potential with respect to the honey samples.
Keywords: Amorpha fruticosa honey volatiles; headspace solid-phase microextraction (HS-SPME); ultrasonic solvent extraction (USE); gas chromatography and mass spectrometry (GC; GC-MS); DPPH scavenging activity Amorpha fruticosa honey volatiles; headspace solid-phase microextraction (HS-SPME); ultrasonic solvent extraction (USE); gas chromatography and mass spectrometry (GC; GC-MS); DPPH scavenging activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Jerković, I.; Marijanović, Z.; Kezić, J.; Gugić, M. Headspace, Volatile and Semi-Volatile Organic Compounds Diversity and Radical Scavenging Activity of Ultrasonic Solvent Extracts from Amorpha fruticosa Honey Samples. Molecules 2009, 14, 2717-2728.

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