Next Article in Journal
Synthesis and Characterization of Photo-Responsive Carbosilane Dendrimers
Next Article in Special Issue
Rhodanineacetic Acid Derivatives as Potential Drugs: Preparation, Hydrophobic Properties and Antifungal Activity of (5-Arylalkylidene-4-oxo-2-thioxo-1,3-thiazolidin-3-yl)acetic Acids
Previous Article in Journal
Phenolics: From Chemistry to Biology
Molecules 2009, 14(6), 2212-2225; doi:10.3390/molecules14062212
Article

Examination of Imprinting Process with Molsidomine as a Template

*  and
Received: 7 May 2009; in revised form: 4 June 2009 / Accepted: 11 June 2009 / Published: 17 June 2009
(This article belongs to the Special Issue ECSOC-12)
Download PDF [252 KB, uploaded 18 June 2014]
Abstract: Eight different functional monomers were used with ethylene glycol dimethacrylate as a cross-linker and molsidomine as a template to obtain molecularly imprinted polymers (MIPs). Non-covalent interactions between molsidomine and each functional monomer in DMSO prior to thermal bulk polymerization were utilized. On the basis of calculated imprinting factors, MIP prepared with N,N’-diallyltartaramide was chosen for further investigations. Examination of interactions in the prepolymerization complex between molsidomine and N,N’-diallyltartaramide was performed using the Job method. The absorbance of isomolar solutions reaching a maximum for the molar ratio of template to monomer equal to 1:4. Scatchard analysis was used for estimation of the dissociation constants and the maximum amounts of binding sites. The polymer based on N,N’-diallyltartaramide has two classes of heterogeneous binding sites characterized by two values of Kd and two Bmax: Kd(1) = 1.17 mM-1 and Bmax(1) = 0.8 μmol/mg for the higher affinity binding sites, and Kd(2) = 200 μM-1 and Bmax(2) = 2.05 μmol/mg for the lower affinity binding sites. Furthermore, effects of pH and organic solvent on binding properties of MIP and NIP were investigated, together with release of molsidomine from both MIP and NIP.
Keywords: molsidomine; molecularly imprinted polymers; sustained release molsidomine; molecularly imprinted polymers; sustained release
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Luliński, P.; Maciejewska, D. Examination of Imprinting Process with Molsidomine as a Template. Molecules 2009, 14, 2212-2225.

AMA Style

Luliński P, Maciejewska D. Examination of Imprinting Process with Molsidomine as a Template. Molecules. 2009; 14(6):2212-2225.

Chicago/Turabian Style

Luliński, Piotr; Maciejewska, Dorota. 2009. "Examination of Imprinting Process with Molsidomine as a Template." Molecules 14, no. 6: 2212-2225.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert