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Open AccessCommunication
Molecules 2009, 14(6), 2043-2048; doi:10.3390/molecules14062043

Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase

1
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China
2
Institute of Agricultural Modernization, Jilin Agricultural University, Changchun, 130118, China
3
School of Resources and Environment, Jilin Agricultural University, Changchun, 130118, China
4
Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China
5
Saskatoon Research Center, Agriculture and Agri-food Canada, Saskatoon, SK S7N 0X2, Canada
6
College of Plant Science, Jilin University, Changchun, 130062, China
These authors contributed equally and are considered as co-first authors
*
Authors to whom correspondence should be addressed.
Received: 6 March 2009 / Revised: 10 May 2009 / Accepted: 31 May 2009 / Published: 8 June 2009
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Abstract

An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (~170 kD) showed the ability to transform ginsenoside Rf into Rh1. View Full-Text
Keywords: Aspergillus niger; β-glucosidase; ginsenoside Rf; ginsenoside Rh1 Aspergillus niger; β-glucosidase; ginsenoside Rf; ginsenoside Rh1
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ruan, C.-C.; Zhang, H.; Zhang, L.-X.; Liu, Z.; Sun, G.-Z.; Lei, J.; Qin, Y.-X.; Zheng, Y.-N.; Li, X.; Pan, H.-Y. Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase. Molecules 2009, 14, 2043-2048.

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