Molecules 2009, 14(6), 2043-2048; doi:10.3390/molecules14062043
Communication

Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase

Received: 6 March 2009; in revised form: 10 May 2009 / Accepted: 31 May 2009 / Published: 8 June 2009
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (~170 kD) showed the ability to transform ginsenoside Rf into Rh1.
Keywords: Aspergillus niger; β-glucosidase; ginsenoside Rf; ginsenoside Rh1
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MDPI and ACS Style

Ruan, C.-C.; Zhang, H.; Zhang, L.-X.; Liu, Z.; Sun, G.-Z.; Lei, J.; Qin, Y.-X.; Zheng, Y.-N.; Li, X.; Pan, H.-Y. Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase. Molecules 2009, 14, 2043-2048.

AMA Style

Ruan C-C, Zhang H, Zhang L-X, Liu Z, Sun G-Z, Lei J, Qin Y-X, Zheng Y-N, Li X, Pan H-Y. Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase. Molecules. 2009; 14(6):2043-2048.

Chicago/Turabian Style

Ruan, Chang-Chun; Zhang, Hao; Zhang, Lian-Xue; Liu, Zhi; Sun, Guang-Zhi; Lei, Jun; Qin, Yu-Xia; Zheng, Yi-Nan; Li, Xiang; Pan, Hong-Yu. 2009. "Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase." Molecules 14, no. 6: 2043-2048.

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