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Effects of a Thiosemicarbazide Camphene Derivative on Trichophyton mentagrophytes
Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, PR, Brazil
Departamento de Química, Universidade Estadual de Maringá, PR, Brazil
* Authors to whom correspondence should be addressed.
Received: 24 March 2009; in revised form: 6 May 2009 / Accepted: 7 May 2009 / Published: 13 May 2009
Abstract: Thiosemicarbazides are compounds known for their biological activity, particularly their antimicrobial properties, which include activity against fungi. The difficulty of treating fungal diseases induced us to assess the antifungal properties of some novel thiosemicarbazide compounds. We selected the natural products limonene and camphene as sources for the preparation of these new thiosemicarbazide derivatives. The compound N(4)-[2,2-dimethyl-3-methylnorbornane]-thiosemicarbazide (TIO C) showed an antifungal effect on Trichophyton mentagrophytes, with values of MIC = 55 mmol L-1 and MFC = 110 mmol L-1. Scanning-electron microscopy showed a decrease in mycelium development and morphological alterations of T. mentagrophytes cultured on nail fragments and treated with TIO C. In an attempt to discover its mode of action, we noted that ergosterol is apparently not a target of TIO C activity. An effect of TIO C on T. mentagrophytes cell walls and dividing cross-walls was shown by observed impairment of the fluorescence of tissues stained with calcofluor white, a specific marker for fungal chitin, suggesting that the compound can affect and damage the cell-wall structure or may interfere with its formation, during cell division, growth, and morphogenesis. This approach to the synthesis of new derivatives might provide interesting compounds with greater biological activity in pharmacological research.
Keywords: antifungal activity; thiosemicarbazide; camphene; Trichophyton mentagrophytes
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MDPI and ACS Style
Yamaguchi, M.U.; Barbosa da Silva, A.P.; Ueda-Nakamura, T.; Dias Filho, B.P.; Conceição da Silva, C.; Nakamura, C.V. Effects of a Thiosemicarbazide Camphene Derivative on Trichophyton mentagrophytes. Molecules 2009, 14, 1796-1807.
Yamaguchi MU, Barbosa da Silva AP, Ueda-Nakamura T, Dias Filho BP, Conceição da Silva C, Nakamura CV. Effects of a Thiosemicarbazide Camphene Derivative on Trichophyton mentagrophytes. Molecules. 2009; 14(5):1796-1807.
Yamaguchi, Mirian Ueda; Barbosa da Silva, Ana Paula; Ueda-Nakamura, Tânia; Dias Filho, Benedito Prado; Conceição da Silva, Cleuza; Nakamura, Celso Vataru. 2009. "Effects of a Thiosemicarbazide Camphene Derivative on Trichophyton mentagrophytes." Molecules 14, no. 5: 1796-1807.