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• Khurram, M
• Khan, M
• Hameed, A
• Abbas, N
• Qayum, A
• Inayat, H
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• Khan, M
• Hameed, A
• Abbas, N
• Qayum, A
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• Khan, M
• Hameed, A
• Abbas, N
• Qayum, A
• Inayat, H

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Molecules 2009, 14(3), 1332-1341; doi:10.3390/molecules14031332

Article

Antibacterial Activities of Dodonaea viscosa using Contact Bioautography Technique

Muhammad Khurram^{1, 2} , Murad Ali Khan^{2, 3,}* , Abdul Hameed^1,*, Naz Abbas² , Abdul Qayum⁴  and Humaira Inayat⁵

¹ 1-Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan ² 2-Medicinal Botanical Centre, PCSIR Laboratories, University Road, Peshawar, Pakistan ³ 3-Department of Chemistry, Kohat University of Science and Technology, Kohat-26000 Pakistan ⁴ 4-Department of Pharmacology, Peshawar Medical College, Warsak road, Peshawar Pakistan ⁵ 5-Institute of Chemical Sciences, University of Peshawar, Peshawar Pakistan

* Authors to whom correspondence should be addressed.

Received: 21 November 2008; in revised form: 18 February 2009 / Accepted: 11 March 2009 / Published: 26 March 2009

Download PDF Full-Text [144 KB, uploaded 26 March 2009 16:31 CET]

Abstract: The crude ethanolic extract and n-hexane, dichloromethane, ethyl acetate, n-butanol and aqueous fractions of Dodonaea viscosa were analyzed for antibacterial potential against four Gram positive bacteria: Bacillus subtilis, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, and three Gram negative bacteria: Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa. Preliminary screening showed inhibition against Staphylococcus aureus, Micrococcus luteus, Escherichia coli and Pseudomonas aeruginosa. The thin layer chromatograms of the fractions were then subjected to contact bioautography, which showed inhibition zone at different R_f values against Bacillus subtilis, Micrococcus luteus, Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa, indicating the presence of antibacterial components. The MIC of each fraction was determined through a 96-well micro-titer plate method. The non-viability of the organisms was ascertained by determining the MBC of the fractions.

Keywords: Dodonaea viscosa; Sapindaceae; Antibacterial; Contact bioautography; MIC; MBC

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