Abstract: The crude ethanolic extract and n-hexane, dichloromethane, ethyl acetate, n-butanol and aqueous fractions of Dodonaea viscosa were analyzed for antibacterial potential against four Gram positive bacteria: Bacillus subtilis, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, and three Gram negative bacteria: Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa. Preliminary screening showed inhibition against Staphylococcus aureus, Micrococcus luteus, Escherichia coli and Pseudomonas aeruginosa. The thin layer chromatograms of the fractions were then subjected to contact bioautography, which showed inhibition zone at different Rf values against Bacillus subtilis, Micrococcus luteus, Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa, indicating the presence of antibacterial components. The MIC of each fraction was determined through a 96-well micro-titer plate method. The non-viability of the organisms was ascertained by determining the MBC of the fractions.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Khurram, M.; Khan, M.A.; Hameed, A.; Abbas, N.; Qayum, A.; Inayat, H. Antibacterial Activities of Dodonaea viscosa using Contact Bioautography Technique. Molecules 2009, 14, 1332-1341.
Khurram M, Khan MA, Hameed A, Abbas N, Qayum A, Inayat H. Antibacterial Activities of Dodonaea viscosa using Contact Bioautography Technique. Molecules. 2009; 14(3):1332-1341.
Khurram, Muhammad; Khan, Murad Ali; Hameed, Abdul; Abbas, Naz; Qayum, Abdul; Inayat, Humaira. 2009. "Antibacterial Activities of Dodonaea viscosa using Contact Bioautography Technique." Molecules 14, no. 3: 1332-1341.