Next Article in Journal
Synthesis and in vitro Evaluation of New Benzothiazole Derivatives as Schistosomicidal Agents
Next Article in Special Issue
Identification of the Products of Oxidation of Quercetin by Air Oxygenat Ambient Temperature
Previous Article in Journal / Special Issue
Characterization of Flavonoid Subgroups and Hydroxy Substitution by HPLC-MS/MS
Molecules 2007, 12(3), 607-621; doi:10.3390/12030607
Article

Methyl Jasmonate and Salicylic Acid Induced Oxidative Stress and Accumulation of Phenolics in Panax ginseng Bioreactor Root Suspension Cultures

1
, 2
 and 2,*
Received: 24 January 2007; in revised form: 13 March 2007 / Accepted: 18 March 2007 / Published: 23 March 2007
(This article belongs to the Special Issue Phenolics and Polyphenolics)
Download PDF [124 KB, uploaded 18 June 2014]
Abstract: To investigate the enzyme variations responsible for the synthesis of phenolics, 40 day-old adventitious roots of Panax ginseng were treated with 200 μM methyl jasmonate (MJ) or salicylic acid (SA) in a 5 L bioreactor suspension culture (working volume 4 L). Both treatments caused an increase in the carbonyl and hydrogen peroxide (H2O2) contents, although the levels were lower in SA treated roots. Total phenolic, flavonoid, ascorbic acid, non-protein thiol (NPSH) and cysteine contents and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical reducing activity were increased by MJ and SA. Fresh weight (FW) and dry weight (DW) decreased significantly after 9 days of exposure to SA and MJ. The highest total phenolics (62%), DPPH activity (40%), flavonoids (88%), ascorbic acid (55%), NPSH (33%), and cysteine (62%) contents compared to control were obtained after 9 days in SA treated roots. The activities of glucose 6-phosphate dehydrogenase, phenylalanine ammonia lyase, substrate specific peroxidases (caffeic acid peroxidase, quercetin peroxidase and ferulic acid peroxidase) were higher in MJ treated roots than the SA treated ones. Increased shikimate dehydrogenase, chlorogenic acid peroxidase and β-glucosidase activities and proline content were observed in SA treated roots than in MJ ones. Cinnamyl alcohol dehydrogenase activity remained unaffected by both MJ and SA. These results strongly indicate that MJ and SA induce the accumulation of phenolic compounds in ginseng root by altering the phenolic synthesis enzymes.
Keywords: Antioxidant capacity; cysteine; phenylpropanoid pathway enzymes; Panax ginseng; phenolics Antioxidant capacity; cysteine; phenylpropanoid pathway enzymes; Panax ginseng; phenolics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Ali, M.B.; Hahn, E.-J.; Paek, K.-Y. Methyl Jasmonate and Salicylic Acid Induced Oxidative Stress and Accumulation of Phenolics in Panax ginseng Bioreactor Root Suspension Cultures. Molecules 2007, 12, 607-621.

AMA Style

Ali MB, Hahn E-J, Paek K-Y. Methyl Jasmonate and Salicylic Acid Induced Oxidative Stress and Accumulation of Phenolics in Panax ginseng Bioreactor Root Suspension Cultures. Molecules. 2007; 12(3):607-621.

Chicago/Turabian Style

Ali, Mohammad B.; Hahn, Eun-Joo; Paek, Kee-Yoeup. 2007. "Methyl Jasmonate and Salicylic Acid Induced Oxidative Stress and Accumulation of Phenolics in Panax ginseng Bioreactor Root Suspension Cultures." Molecules 12, no. 3: 607-621.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert