Next Article in Journal / Special Issue
Methyl Jasmonate and Salicylic Acid Induced Oxidative Stress and Accumulation of Phenolics in Panax ginseng Bioreactor Root Suspension Cultures
Previous Article in Journal
Proteasome and NF-κB Inhibiting Phaeophytins from the Green Alga Cladophora fascicularis
Previous Article in Special Issue
Phenolic Content and Antioxidant Properties of Soybean (Glycine max (L.) Merr.) Seeds
Molecules 2007, 12(3), 593-606; doi:10.3390/12030593

Characterization of Flavonoid Subgroups and Hydroxy Substitution by HPLC-MS/MS

1,* , 2, 2 and 1,*
1 National Technical University of Athens, School of Chemical Engineering, 9 Iroon Polytehniou st, Zografou Campus, 157 80, Athens, Greece 2 Biomedical Sciences Research Center “Alexander Fleming”, 34 Fleming st, 16672, Vari, Greece
* Authors to whom correspondence should be addressed.
Received: 15 January 2007 / Revised: 13 March 2007 / Accepted: 14 March 2007 / Published: 21 March 2007
(This article belongs to the Special Issue Phenolics and Polyphenolics)
View Full-Text   |   Download PDF [96 KB, uploaded 18 June 2014]   |  


HPLC-DAD coupled with mass spectrometry in the positive ionization mode was applied to study the fragmentation of twelve selected flavonoids. Compounds belonging to all the major subgroups found in common plants, i.e. flavonols, flavones, dihydroflavonols, flavanones and flavanols were studied. Compound standards were injected into the spectrometer and produced characteristic mass spectra. The fragmentation of each compound was studied and it was shown that the dehydration andcarbon monoxide losses from the [M+H]+ ion by the members of each subgroup produced specific fragments, thus allowing the characterization of the flavonoid subgroups. Moreover, fragments resulting from fission of the C-rings are specific of each subgroup and revealed the substitution pattern of A- and B-rings. In order to verify the identifying efficiency of the positive ionization mode through these characteristic fragmentations, the unknown flavonoids of an Origanum vulgare diethyl ether extract were separated withthe HPLC system and the major peaks were successfully identified with the mass spectrometer.
Keywords: HPLC-MS/MS analysis; flavonoids; Origanum vulgare HPLC-MS/MS analysis; flavonoids; Origanum vulgare
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Tsimogiannis, D.; Samiotaki, M.; Panayotou, G.; Oreopoulou, V. Characterization of Flavonoid Subgroups and Hydroxy Substitution by HPLC-MS/MS. Molecules 2007, 12, 593-606.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert