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Entropy 2012, 14(3), 581-598; doi:10.3390/e14030581
Review

Application of Solution NMR Spectroscopy to Study Protein Dynamics

1
 and 2,*
Received: 30 January 2012; in revised form: 13 March 2012 / Accepted: 16 March 2012 / Published: 22 March 2012
(This article belongs to the Special Issue Loop Entropy)
Download PDF [1541 KB, uploaded 22 March 2012]
Abstract: Recent advances in spectroscopic methods allow the identification of minute fluctuations in a protein structure. These dynamic properties have been identified as keys to some biological processes. The consequences of this structural flexibility can be far‑reaching and they add a new dimension to the structure-function relationship of biomolecules. Nuclear Magnetic Resonance (NMR) spectroscopy allows the study of structure as well as dynamics of biomolecules in a very broad range of timescales at atomic level. A number of new NMR methods have been developed recently to allow the measurements of time scales and spatial fluctuations, which in turn provide the thermodynamics associated with the biological processes. Since NMR parameters reflect ensemble measurements, structural ensemble approaches in analyzing NMR data have also been developed. These new methods in some instances can even highlight previously hidden conformational features of the biomolecules. In this review we describe several solution NMR methods to study protein dynamics and discuss their impact on important biological processes.
Keywords: protein; protein structure; protein dynamics; protein interaction; solution NMR spectroscopy protein; protein structure; protein dynamics; protein interaction; solution NMR spectroscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Göbl, C.; Tjandra, N. Application of Solution NMR Spectroscopy to Study Protein Dynamics. Entropy 2012, 14, 581-598.

AMA Style

Göbl C, Tjandra N. Application of Solution NMR Spectroscopy to Study Protein Dynamics. Entropy. 2012; 14(3):581-598.

Chicago/Turabian Style

Göbl, Christoph; Tjandra, Nico. 2012. "Application of Solution NMR Spectroscopy to Study Protein Dynamics." Entropy 14, no. 3: 581-598.


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