Cell-Free Synthetic Biology

doi:10.3390/books978-3-03928-023-0

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Cell-Free Synthetic Biology

Edited by

January 2020

152 pages

ISBN978-3-03928-022-3 (Paperback)
ISBN978-3-03928-023-0 (PDF)

https://doi.org/10.3390/books978-3-03928-023-0

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This book is a reprint of the Special Issue Cell-Free Synthetic Biology that was published in

Biology & Life Sciences

Chemistry & Materials Science

Public Health & Healthcare

Summary

Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas.

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Paperback

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Keywords

cell-free protein synthesis; unnatural amino acid; unnatural protein; cell-free protein synthesis (CFPS); in vitro transcription-translation (TX-TL); cell-free protein expression (CFPE); in vitro protein synthesis; cell-free synthetic biology; cell-free metabolic engineering (CFME); colicins; cell-free protein synthesis; antimicrobials; chaperones; in vitro translation; protein production; drug development; cell-free protein synthesis; eukaryotic lysates; microsomes; growth factors; enzymes; cell-free metabolic engineering; cell-free protein synthesis; bioconversion platform; synthetic biology; cell-free transcription-translation; rapid prototyping; artificial cell; riboregulator; DNA origami; mathematical model; cell-free protein synthesis; CFPS; tPa; tissue plasminogen activator; ischemic stroke; cell-free protein synthesis; E. coli crude extract preparation; genomically engineered E. coli; sonication; n/a