Role and Functional Domain of Hepatitis B Virus X Protein in Regulating HBV Transcription and Replication in Vitro and in Vivo
Abstract
:1. Introduction
2. Results
2.1. Effect of HBx on Wild-Type HBV Transcription and Replication in HepG2 and Huh7 Cells
2.2. Effect of HBx on Wild-Type HBV Transcription and Replication in Vivo
2.3. Role of the C-terminal Transactivation Domain of HBx in HBV Transcription and Replication in Vivo
2.4. Role of Different HBx Regions in HBV Transcription and Replication Regulation in Vivo
3. Discussion
4. Experimental
4.1. Plasmids
4.2. Cell Culture and Plasmid Transfection in Vitro
4.3. Injection of Naked Plasmid DNA in Vivo
4.4. Analysis of HBV RNA and HBV DNA Replication Intermediates
5. Conclusions
Acknowledgments
Conflict of Interest
References and Notes
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Gong, D.-Y.; Chen, E.-Q.; Huang, F.-J.; Leng, X.-H.; Cheng, X.; Tang, H. Role and Functional Domain of Hepatitis B Virus X Protein in Regulating HBV Transcription and Replication in Vitro and in Vivo. Viruses 2013, 5, 1261-1271. https://doi.org/10.3390/v5051261
Gong D-Y, Chen E-Q, Huang F-J, Leng X-H, Cheng X, Tang H. Role and Functional Domain of Hepatitis B Virus X Protein in Regulating HBV Transcription and Replication in Vitro and in Vivo. Viruses. 2013; 5(5):1261-1271. https://doi.org/10.3390/v5051261
Chicago/Turabian StyleGong, Dao-Yin, En-Qiang Chen, Fei-Jun Huang, Xiao-Hua Leng, Xing Cheng, and Hong Tang. 2013. "Role and Functional Domain of Hepatitis B Virus X Protein in Regulating HBV Transcription and Replication in Vitro and in Vivo" Viruses 5, no. 5: 1261-1271. https://doi.org/10.3390/v5051261