Genome-Wide Identification, Expression Pattern Analysis and Evolution of the Ces/Csl Gene Superfamily in Pineapple (Ananas comosus)
Abstract
:1. Introduction
2. Results
2.1. Identification of Ces /Csl Genes in Pineapple
2.2. Phylogenetic Analysis of the Pineapple Ces/Csl Gene Superfamily
2.3. Gene Structure Analysis and Conserved Motif Identification
2.4. Synteny Analysis of Pineapple Ces/Csl Genes
2.5. Expression Patterns of AcoCes/Csl Genes in Four Different Tissues
2.6. Expression of AcoCes/Csl Genes during Gametophyte Development
3. Discussion
4. Materials and Methods
4.1. Identification of Ces/Csl in Pineapple
4.2. Physicochemical Properties and Phylogenetic Analysis
4.3. Conserved Motifs Analysis of Pineapple Ces/Csl Protein
4.4. Chromosome Localization and Gene Structural Analysis of Pineapple Ces/Csl Genes
4.5. Synteny Analysis of Pineapple Ces/Csl Genes
4.6. RNA-Seq and qRT-PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
Aco | Ananas comosus |
At | Arabidopsis thaliana |
Ces | Cellulose synthase |
Csl | Cellulose synthase-like |
References
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Gene Name | Gene ID | Chr | Length | Amino Acids | Introns | Exons | Isoelectric Point (pI) | Molecular Weight (MW) |
---|---|---|---|---|---|---|---|---|
AcoCesA1 | Aco014283 | LG05 | 7376 | 1104 | 13 | 14 | 6.72 | 122.4 |
AcoCesA2 | Aco018229 | LG01 | 8424 | 1077 | 13 | 14 | 7.92 | 120.7 |
AcoCesA3 | Aco024230 | LG09 | 12,555 | 1081 | 12 | 13 | 8.31 | 119.6 |
AcoCesA4 | Aco014585 | LG08 | 7332 | 1070 | 11 | 12 | 7.61 | 119.9 |
AcoCesA5 | Aco018552 | LG06 | 6358 | 1102 | 13 | 14 | 7.04 | 123.2 |
AcoCesA7 | Aco012076 | LG02 | 6278 | 1062 | 12 | 13 | 6.33 | 118.9 |
AcoCesA8 | Aco006039 | LG16 | 6951 | 1003 | 12 | 13 | 5.93 | 111.7 |
AcoCslA1 | Aco001096 | LG02 | 14,534 | 1126 | 19 | 19 | 8.99 | 127.2 |
AcoCslA2 | Aco006900 | LG22 | 4546 | 536 | 8 | 9 | 9.19 | 60.9 |
AcoCslA3 | Aco001095 | LG02 | 5559 | 560 | 9 | 10 | 8.8 | 62.9 |
AcoCslA4 | Aco004149 | LG15 | 20,041 | 1184 | 20 | 20 | 8.8 | 133.1 |
AcoCslA6 | Aco002889 | LG06 | 9648 | 555 | 15 | 9 | 8.56 | 63 |
AcoCslA9 | Aco016682 | LG17 | 9750 | 607 | 10 | 10 | 9.16 | 68.4. |
AcoCslA11 | Aco014689 | LG20 | 7773 | 568 | 9 | 10 | 7.83 | 64.1 |
AcoCslC1 | Aco004974 | LG07 | 3939 | 727 | 4 | 5 | 8.4 | 81.1 |
AcoCslC4 | Aco000968 | LG02 | 2630 | 673 | 4 | 5 | 8.85 | 77.1 |
AcoCslC5 | Aco008242 | LG19 | 4607 | 747 | 8 | 6 | 8.86 | 84.7 |
AcoCslC6 | Aco013494 | LG20 | 5582 | 709 | 4 | 5 | 8.67 | 78.7 |
AcoCslC8 | Aco011603 | LG06 | 3136 | 663 | 4 | 5 | 8.99 | 74.8 |
AcoCslC12 | Aco008598 | LG09 | 3881 | 773 | 6 | 7 | 6.15 | 85.7 |
AcoCslD1 | Aco004435 | LG05 | 4309 | 1068 | 4 | 5 | 7.62 | 116.9 |
AcoCslD2 | Aco015969 | LG22 | 5627 | 1174 | 4 | 3 | 7.49 | 129.2 |
AcoCslD3 | Aco030607 | scaffold_1556 | 5631 | 1131 | 4 | 3 | 6.74 | 124.7 |
AcoCslD4 | Aco017129 | LG14 | 6362 | 1159 | 6 | 5 | 6.46 | 127.5 |
AcoCslD5 | Aco013738 | LG13 | 3953 | 1201 | 2 | 3 | 8.45 | 130.9 |
AcoCslD6 | Aco016995 | LG08 | 4094 | 1193 | 4 | 5 | 7.9 | 130.5 |
AcoCslD7 | Aco025070 | LG01 | 7403 | 1170 | 3 | 4 | 6.7 | 128.9 |
AcoCslD8 | Aco017291 | LG03 | 3892 | 1185 | 2 | 3 | 7.08 | 130.2 |
AcoCslE1 | Aco026335 | LG19 | 5869 | 680 | 7 | 8 | 6.99 | 76.2 |
AcoCslE2 | Aco000884 | LG02 | 4750 | 736 | 7 | 8 | 8.02 | 81.9 |
AcoCslG1 | Aco013153 | LG24 | 47,982 | 1615 | 16 | 17 | 7.92 | 178.6 |
AcoCslJ1 | Aco013513 | LG20 | 13,869 | 807 | 9 | 10 | 8.91 | 89.2 |
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Cao, S.; Cheng, H.; Zhang, J.; Aslam, M.; Yan, M.; Hu, A.; Lin, L.; Ojolo, S.P.; Zhao, H.; Priyadarshani, S.V.G.N.; et al. Genome-Wide Identification, Expression Pattern Analysis and Evolution of the Ces/Csl Gene Superfamily in Pineapple (Ananas comosus). Plants 2019, 8, 275. https://doi.org/10.3390/plants8080275
Cao S, Cheng H, Zhang J, Aslam M, Yan M, Hu A, Lin L, Ojolo SP, Zhao H, Priyadarshani SVGN, et al. Genome-Wide Identification, Expression Pattern Analysis and Evolution of the Ces/Csl Gene Superfamily in Pineapple (Ananas comosus). Plants. 2019; 8(8):275. https://doi.org/10.3390/plants8080275
Chicago/Turabian StyleCao, Shijiang, Han Cheng, Jiashuo Zhang, Mohammad Aslam, Maokai Yan, Anqi Hu, Lili Lin, Simon Peter Ojolo, Heming Zhao, S.V.G.N. Priyadarshani, and et al. 2019. "Genome-Wide Identification, Expression Pattern Analysis and Evolution of the Ces/Csl Gene Superfamily in Pineapple (Ananas comosus)" Plants 8, no. 8: 275. https://doi.org/10.3390/plants8080275