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Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV, T. gondii, and H. pylori
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High-Throughput 2017, 6(4), 15; doi:10.3390/ht6040015

Study of the Humoral Immune Response towards HCV Genotype 4 Using a Bead-Based Multiplex Serological Assay

NMI Natural and Medical Sciences Institute at the University of Tuebingen, 72770 Reutlingen, Germany
Immunobiology of Dendritic Cells, Institute Pasteur, 75015 Paris, France
Inserm U1223, Institute Pasteur, 75015 Paris, France
Centre for Translational Research, Institute Pasteur, 75015 Paris, France
Unité d’hépatologie, Groupe Hospitalier Cochin Hôtel-Dieu, Université René Descartes, 75006 Paris, France
Department of Microbiology, Faculty of Medicine, Minia University, Minia 11432, Egypt
Endemic Medicine and Hepatogastroenterology, Faculty of Medicine, Cairo University, Cairo 12613, Egypt
Emerging Disease Epidemiology Unit, Institute Pasteur, 75015 Paris, France
Conservatoire National des Arts et Métiers, Unité PACRI, 75003 Paris, France
Author to whom correspondence should be addressed.
Academic Editors: Xiaobo Yu and Joshua LaBaer
Received: 31 August 2017 / Revised: 26 September 2017 / Accepted: 23 October 2017 / Published: 30 October 2017
(This article belongs to the Special Issue Protein Microarrays)
View Full-Text   |   Download PDF [1686 KB, uploaded 1 November 2017]   |  


Hepatitis C is one of the leading causes of hepatocellular carcinoma and remains at a high prevalence in Egypt and other resource-limited countries. Several hepatitis C virus (HCV) genotypes are distributed throughout the world, with genotype 4 being most common in North and Central Africa. We developed a multiplex serological assay for the detection of the HCV specific humoral immune response, with a focus on genotype 4. For the multiplex HCV assay we used twelve antigenic regions of different HCV proteins (core, and non-structural (NS) proteins NS3, NS4, NS5A, NS5B) and validated the assay technically and clinically. In comparison to a commercially available test, our assay revealed a higher sensitivity for genotype 4, and is therefore more suited for studying immune seroconversion in samples from acutely infected Egyptian HCV patients. Furthermore, our assay discriminates acutely and chronically infected HCV patients. Of 296 well characterized HCV patient samples, 83.9% of the acute samples and 86.5% of the chronic samples could be correctly classified. In sum, this newly developed serological HCV assay has a higher sensitivity for HCV genotype 4, and can thus improve diagnostic accuracy. Through the discrimination of acutely and chronically infected HCV patients the assay may be useful in supporting clinical management of HCV patients. View Full-Text
Keywords: hepatitis C virus genotype 4; HCV; multiplex serological assay; antibody response; acute or chronic hepatitis C virus infection hepatitis C virus genotype 4; HCV; multiplex serological assay; antibody response; acute or chronic hepatitis C virus infection

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Filomena, A.; Göpfert, J.C.; Duffy, D.; Pol, S.; Abdel-Hamid, M.; Esmat, G.; Fontanet, A.; Albert, M.L.; Joos, T.O.; Schneiderhan-Marra, N. Study of the Humoral Immune Response towards HCV Genotype 4 Using a Bead-Based Multiplex Serological Assay. High-Throughput 2017, 6, 15.

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