Next Article in Journal
A Computational Workflow Translates a 58-Gene Signature to a Formalin-Fixed, Paraffin-Embedded Sample-Based Companion Diagnostic for Personalized Treatment of the BRAF-Mutation-Like Subtype of Colorectal Cancers
Next Article in Special Issue
Development and Validation of an Ultrasensitive Procalcitonin Sandwich Immunoassay
Previous Article in Journal / Special Issue
Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV, T. gondii, and H. pylori
Article Menu

Export Article

Open AccessArticle
High-Throughput 2017, 6(4), 15; doi:10.3390/ht6040015

Study of the Humoral Immune Response towards HCV Genotype 4 Using a Bead-Based Multiplex Serological Assay

1
NMI Natural and Medical Sciences Institute at the University of Tuebingen, 72770 Reutlingen, Germany
2
Immunobiology of Dendritic Cells, Institute Pasteur, 75015 Paris, France
3
Inserm U1223, Institute Pasteur, 75015 Paris, France
4
Centre for Translational Research, Institute Pasteur, 75015 Paris, France
5
Unité d’hépatologie, Groupe Hospitalier Cochin Hôtel-Dieu, Université René Descartes, 75006 Paris, France
6
Department of Microbiology, Faculty of Medicine, Minia University, Minia 11432, Egypt
7
Endemic Medicine and Hepatogastroenterology, Faculty of Medicine, Cairo University, Cairo 12613, Egypt
8
Emerging Disease Epidemiology Unit, Institute Pasteur, 75015 Paris, France
9
Conservatoire National des Arts et Métiers, Unité PACRI, 75003 Paris, France
*
Author to whom correspondence should be addressed.
Academic Editors: Xiaobo Yu and Joshua LaBaer
Received: 31 August 2017 / Revised: 26 September 2017 / Accepted: 23 October 2017 / Published: 30 October 2017
(This article belongs to the Special Issue Protein Microarrays)
View Full-Text   |   Download PDF [1686 KB, uploaded 1 November 2017]   |  

Abstract

Hepatitis C is one of the leading causes of hepatocellular carcinoma and remains at a high prevalence in Egypt and other resource-limited countries. Several hepatitis C virus (HCV) genotypes are distributed throughout the world, with genotype 4 being most common in North and Central Africa. We developed a multiplex serological assay for the detection of the HCV specific humoral immune response, with a focus on genotype 4. For the multiplex HCV assay we used twelve antigenic regions of different HCV proteins (core, and non-structural (NS) proteins NS3, NS4, NS5A, NS5B) and validated the assay technically and clinically. In comparison to a commercially available test, our assay revealed a higher sensitivity for genotype 4, and is therefore more suited for studying immune seroconversion in samples from acutely infected Egyptian HCV patients. Furthermore, our assay discriminates acutely and chronically infected HCV patients. Of 296 well characterized HCV patient samples, 83.9% of the acute samples and 86.5% of the chronic samples could be correctly classified. In sum, this newly developed serological HCV assay has a higher sensitivity for HCV genotype 4, and can thus improve diagnostic accuracy. Through the discrimination of acutely and chronically infected HCV patients the assay may be useful in supporting clinical management of HCV patients. View Full-Text
Keywords: hepatitis C virus genotype 4; HCV; multiplex serological assay; antibody response; acute or chronic hepatitis C virus infection hepatitis C virus genotype 4; HCV; multiplex serological assay; antibody response; acute or chronic hepatitis C virus infection
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Filomena, A.; Göpfert, J.C.; Duffy, D.; Pol, S.; Abdel-Hamid, M.; Esmat, G.; Fontanet, A.; Albert, M.L.; Joos, T.O.; Schneiderhan-Marra, N. Study of the Humoral Immune Response towards HCV Genotype 4 Using a Bead-Based Multiplex Serological Assay. High-Throughput 2017, 6, 15.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
High-Throughput EISSN 2571-5135 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top