Development and Validation of an Ultrasensitive Procalcitonin Sandwich Immunoassay
AbstractProcalcitonin (PCT) is well established as a highly specific biomarker for the detection of bacterial infections and sepsis. However, the currently available diagnostic tests are not able to detect very low or very early increases of PCT or even baseline levels in healthy individuals or patients with non-bacterial infections. In order to be able to detect these very low concentrations of PCT, a sandwich immunoassay was developed using high sensitivity Single Molecule Array technology (Simoa). The assay was thoroughly validated and applied to analyze human cerebrospinal fluid (CSF) and serum samples from patients with bacterial or viral meningitis as well as CSF, serum, and K2 EDTA plasma from healthy control subjects. A 50-fold increase in sensitivity compared to the current gold standard assays was achieved, which was sensitive enough for the detection of baseline PCT levels. Both serum and CSF showed significantly elevated PCT levels in patients with bacterial meningitis compared to patients with viral meningitis and the healthy control group. Procalcitonin concentration levels for patients with viral meningitis and the control group could be measured, but were not significantly different. The determination of PCT in the low pg·mL−1 range could help to improve the monitoring of bacterial infectious diseases, as PCT level changes could be detected earlier. View Full-Text
- Supplementary File 1:
Supplementary (PDF, 278 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Carcamo Yañez, V.A.; Göpfert, J.C.; Otto, M.; Tumani, H.; Peter, A.; Joos, T.O. Development and Validation of an Ultrasensitive Procalcitonin Sandwich Immunoassay. High-Throughput 2017, 6, 18.
Carcamo Yañez VA, Göpfert JC, Otto M, Tumani H, Peter A, Joos TO. Development and Validation of an Ultrasensitive Procalcitonin Sandwich Immunoassay. High-Throughput. 2017; 6(4):18.Chicago/Turabian Style
Carcamo Yañez, Viviana A.; Göpfert, Jens C.; Otto, Markus; Tumani, Hayrettin; Peter, Andreas; Joos, Thomas O. 2017. "Development and Validation of an Ultrasensitive Procalcitonin Sandwich Immunoassay." High-Throughput 6, no. 4: 18.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.