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Proceedings 2017, 1(4), 519; doi:10.3390/proceedings1040519

Bacillus spp. Cells Captured Selectively by Phages and Identified by Surface Enhanced Raman Spectroscopy Technique

1
ENEA, Italian Agency for New Technologies, Energy and Sustainable Development, Via Enrico Fermi 45, I-00044 Frascati, Italy
2
Creo, Electro Optics Research Consortium, SS 17 Località Boschetto, I-67100 L’Aquila, Italy
3
University Claude Bernard, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Lyon 1, Bâtiment CPE 43, bd du 11 novembre 1918, 69622 Villeurbanne, France
4
Military University of Technology, Institute of Optoelectronics ul. gen. Sylwestra Kaliskiego 2, 00-908 Warsaw, Poland
5
Leonardo—S.p.A. Via Tiburtina km 12,400, 00131 Rome, Italy
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
*
Author to whom correspondence should be addressed.
Published: 17 August 2017
Download PDF [1005 KB, uploaded 17 August 2017]

Abstract

Surface Enhanced Raman Spectroscopy (SERS), has been employed as a rapid and accurate tool for recognition and classification of different microorganisms. The vibrational spectrum intrinsically provides the fingerprint of the molecular structure of microorganisms, without sample preparation. The characterization of Bacillus spp., used as a simulants of the dangerous Bacillus anthracis by means of SERS technique has been carried out. The positive results on the use of the SERS substrate functionalized with phages specific for bacteria are showed. This work is a part of the RAMBO project (Rapid Air particle Monitoring against BiOlogical threats) funded by EDA (European Defense Agency) whose goal is the development of an advanced “detect to warn sensor” of cells and spores of dangerous bacteria, with high performance, good selectivity and reliability.
Keywords: sensors; SERS; bacteria; phages; Bacillus spp. sensors; SERS; bacteria; phages; Bacillus spp.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lai, A.; Almaviva, S.; Spizzichino, V.; Luciani, D.; Palucci, A.; Mengali, S.; Marquette, C.; Berthuy, O.; Jankiewicz, B.; Pierno, L. Bacillus spp. Cells Captured Selectively by Phages and Identified by Surface Enhanced Raman Spectroscopy Technique. Proceedings 2017, 1, 519.

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