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Open AccessCommunication
Horticulturae 2016, 2(3), 10; doi:10.3390/horticulturae2030010

Encapsulation of Shoot Tips and Nodal Segments for in Vitro Storage of “Kober 5BB” Grapevine Rootstock

Trees and Timber Institute (IVALSA-Istituto per la Valorizzazione del Legno e delle Specie Arboree), National Research Council, 50019 Florence, Italy
Academic Editors: Astrid Forneck and Douglas D. Archbold
Received: 22 March 2016 / Revised: 5 August 2016 / Accepted: 9 August 2016 / Published: 11 August 2016
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Abstract

In vitro preservation of the “Kober 5BB” rootstock (Vitis berlandieri × Vitis riparia) was assessed with the encapsulation technique and slow growth storage. Shoot tips and nodal segments excised from in vitro cultures were encapsulated in calcium-alginate beads. A 30 min ion exchange time proved optimal for forming proper beads. The encapsulated and naked explants were stored at 4 °C in the dark or light. After 9 months of cold storage, the highest regrowth, 83.3%, was recorded for the encapsulated shoot tips maintained in darkness. The development of the encapsulated nodal segments was 55.6% under the same storage conditions. The encapsulated explants had a better regrowth capacity after storage than the naked explants. View Full-Text
Keywords: alginate bead; grapevine rootstock; in vitro culture; slow growth storage alginate bead; grapevine rootstock; in vitro culture; slow growth storage
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Benelli, C. Encapsulation of Shoot Tips and Nodal Segments for in Vitro Storage of “Kober 5BB” Grapevine Rootstock. Horticulturae 2016, 2, 10.

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