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Fermentation 2017, 3(4), 58; doi:10.3390/fermentation3040058

A Microtiter Plate Assay as a Reliable Method to Assure the Identification and Classification of the Veil-Forming Yeasts during Sherry Wines Ageing

Department of Biomedicine, Biotechnology and Public Health, University of Cádiz, Cádiz 11510, Spain
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Received: 10 October 2017 / Revised: 27 October 2017 / Accepted: 31 October 2017 / Published: 3 November 2017
(This article belongs to the Special Issue Yeast Biotechnology 2.0)
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Abstract

Yeasts involved in veil formation during biological ageing of Sherry wines are mainly Saccharomyces cerevisiae, and they have traditionally been divided into four races or varieties: beticus, cheresiensis, montuliensis and rouxii. Recent progress in molecular biology has led to the development of several techniques for yeast identification, based on similarity or dissimilarity of DNA, RNA or proteins. In view of the latest yeast taxonomy, there are no more races. However, molecular techniques are not enough to understand the real veil-forming yeast diversity and dynamics in Sherry wines. We propose a reliable method, using a microtiter reader, to evaluate the fermentation and assimilation of carbon and nitrogen sources, the osmotolerance and the antibiotic resistance, using 18 S. cerevisiae and 5 non-Saccharomyces yeast strains, to allow correct identification and classification of the yeast strains present in the velum of flor complex. View Full-Text
Keywords: flor yeast; microplate; fermentation; assimilation; Saccharomyces cerevisiae; non-Saccharomyces; velum of flor flor yeast; microplate; fermentation; assimilation; Saccharomyces cerevisiae; non-Saccharomyces; velum of flor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ruíz-Muñoz, M.; Bernal-Grande, M.C.; Cordero-Bueso, G.; González, M.; Hughes-Herrera, D.; Cantoral, J.M. A Microtiter Plate Assay as a Reliable Method to Assure the Identification and Classification of the Veil-Forming Yeasts during Sherry Wines Ageing. Fermentation 2017, 3, 58.

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