Next Article in Journal
Phenols Removal from Hemicelluloses Pre-Hydrolysate by Laccase to Improve Butanol Production
Next Article in Special Issue
Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938
Previous Article in Journal
Fortification and Elevated Alcohol Concentration Affect the Concentration of Rotundone and Volatiles in Vitis vinifera cv. Shiraz Wine
Previous Article in Special Issue
Succinic Acid: Technology Development and Commercialization
Article Menu
Issue 3 (September) cover image

Export Article

Open AccessFeature PaperArticle
Fermentation 2017, 3(3), 30; doi:10.3390/fermentation3030030

Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures

1
Department of Chemical and Paper Engineering, Western Michigan University, Kalamazoo, MI 49008, USA
2
Department of Chemical Engineering, University of the Philippines Los Baños, College, Laguna 4031, Philippines
*
Author to whom correspondence should be addressed.
Received: 25 May 2017 / Revised: 19 June 2017 / Accepted: 26 June 2017 / Published: 30 June 2017
(This article belongs to the Special Issue Carboxylic Acid Production)
View Full-Text   |   Download PDF [829 KB, uploaded 30 June 2017]   |  

Abstract

Conventional bio-based succinic acid production involves anaerobic bacterial fermentation of pure sugars. This study explored a new route for directly producing succinic acid from minimally-pretreated lignocellulosic biomass via a consolidated bioprocessing technology employing a mixed lignocellulolytic and acidogenic fungal co-culture. The process involved a solid-state pre-fermentation stage followed by a two-phase slurry fermentation stage. During the solid-state pre-fermentation stage, Aspergillus niger and Trichoderma reesei were co-cultured in a nitrogen-rich substrate (e.g., soybean hull) to induce cellulolytic enzyme activity. The ligninolytic fungus Phanerochaete chrysosporium was grown separately on carbon-rich birch wood chips to induce ligninolytic enzymes, rendering the biomass more susceptible to cellulase attack. The solid-state pre-cultures were then combined in a slurry fermentation culture to achieve simultaneous enzymatic cellulolysis and succinic acid production. This approach generated succinic acid at maximum titers of 32.43 g/L after 72 h of batch slurry fermentation (~10 g/L production), and 61.12 g/L after 36 h of addition of fresh birch wood chips at the onset of the slurry fermentation stage (~26 g/L production). Based on this result, this approach is a promising alternative to current bacterial succinic acid production due to its minimal substrate pretreatment requirements, which could reduce production costs. View Full-Text
Keywords: consolidated bioprocessing; fungi; solid-phase fermentation; bio-based chemicals; lignocellulose biomass; mixed cultures consolidated bioprocessing; fungi; solid-phase fermentation; bio-based chemicals; lignocellulose biomass; mixed cultures
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Alcantara, J.; Mondala, A.; Hughey, L.; Shields, S. Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures. Fermentation 2017, 3, 30.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Fermentation EISSN 2311-5637 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top